[Histonet] cryojane question

[Liz Chlipala]

Emily

You can put more than one section on a slide if you need to, but in our
experience it does not work as nicely as depicted all of the time, it
can be a bit tricky to work with on undecalcifed bone and harder
tissues.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
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Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily
Sours
Sent: Friday, June 18, 2010 8:10 AM
To: Kim Merriam; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] cryojane question

Histonetters,

I just looked up what a cryojane was, and it's pretty neat!
Does anyone else use this?
The one flaw seems to be that you can only put one section on a slide
(or at
least that the way it's depicted here:
http://www.instrumedics.com/cryojanetapetransferprocess.htm )
which makes it pretty time consuming.  Also does the uv step interfere
with
in situ protocols? I guess not since the DNA/RNA is already transcribed
and
fixed and therefore wouldn't be mutated.


Emily


Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
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