[Histonet] (no subject)

[Rena Fail]

Are you washing the bone marrows before processing? Years ago ;) when I used acidic zenkers we washed the BM before processing then post fixed in formalin on the processor. The overnight processing was formalin until the machine started 1 hour in 70% ethanol, an hour in 3 changes 95% ethanol, 3, changes 100% ethanol, 3 changes paraffin. for rush processing it was 20 minutes in each. THe bone marrows were cut at 3 microns. i didn't have a problem with tissue washing off. 
Rena Fail



----- Original Message ----
From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wed, June 16, 2010 4:53:22 PM
Subject: [Histonet] (no subject)

Does anyone know a good overnight processing protocol for bone marrows
fixed in Acid Zinc Formalin? My tissue is washing off the slides. I have
tried adhesive slides and they do not help. I am currently using a
processing protocol that many use for bone marrows. I think the AZF may
be dehydrating the tissue and this process protocol is making it worse.
Any suggestions for a protocol? I am not able to change fixatives. 


Thanks,

Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office:  913-234-0576
Fax:  913-433-7639
Email:  Nacaela.Johnson <@t> USOncology.com

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