[Histonet] Non-Glyoxal based formalin substitutes

[Jeff Birkner]

I am wondering what types of Non-glyoxal based formalin substitutes
folks are using.  We currently use Prefer (glyoxal based fixative) as
our formalin substitute.  We are very satisfied with this product for
most cases; however, we are having problems with inflammatory skin
cases.  We need to be able to accurately evaluate the absence or
presence and relative density of eosinophils and the absence or presence
of extravasated red blood cells to differentiate between otherwise
similar inflammatory skin diseases.  Prefer (glyoxal) binds to the
argine site which inhibits eosin staining of eosinophils and it also
lyses erythrocytes.  Are there any formalin free substitutes that will
work for this situation? 

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner <@t> colabserv.com

 


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