[Histonet] RE: Histonet Digest, Vol 79, Issue 11

Fri Jun 11 12:57:38 CDT 2010

NSH has the Histodeck Flash Cards available for sale.  
Visit our website  https://www.nshonline.org/eweb/DynamicPage.aspx?expires=yes&webcode=COEPubSearch
to purchase.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, June 11, 2010 1:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 11

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Today's Topics:

   1. RE: Histodeck Flash Cards (Dave Johnson)
   2. Thin Prep (Cindy DuBois)
   3. Full Time HT in E. Tennessee (rgrow <@t> bmnet.com)
   4. Re: Ocular fiative (Joseph Saby)
   5. Job Opening  (Kaye Ryan)
   6. Can you do Masson's trichrome on frozen sections (jessamine winer)
   7. RE: SPAM-LOW:  [Histonet] ink on film coverslips (Patsy Ruegg)

----------------------------------------------------------------------

Message: 1
Date: Thu, 10 Jun 2010 13:12:11 -0400
From: "Dave Johnson" <djohnson <@t> mercedesmedical.com>
Subject: RE: [Histonet] Histodeck Flash Cards
To: "Burton, Lynn" <Lynn.Burton <@t> Illinois.gov>,	"Kim Merriam"
	<kmerriam2003 <@t> yahoo.com>, <laurie <@t> conxis.com>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F7CEA5A181F69F4C84A352BD311BCEBD03E98D94 <@t> mail1.mercedesmedical.com>
Content-Type: text/plain;	charset="us-ascii"

http://www.ascp.org/ASCPStore/Store/Books/5930.aspx

Authors: Freida L. Carson and Christa Hladik

Designed for quick and easy self-study for the ASCP HTL certifying
examination, HistoDeck flash cards include questions structured like
those on the exam. The cards cover content based on the new third
edition of Freida Carson's classic Histotechnology volume and include
additional images not included in the book. If you use flash cards for
exam prep, this is the study tool for you.

Contents:
Fixation - 38 questions, 13 images
Immunohistochemistry - 30 questions, 6 images
Instrumentation - 20 questions, 15 images
Processing - 19 questions, 10 images
Routine staining - 33 questions, 17 images
Safety - 32 questions, 5 images
Special staining - 74 questions, 39 images
Miscellaneous - 34 questions, 6 images

400 cards * 111 images * 2009
ISBN: 9780891895930 * Order # 5930

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burton,
Lynn
Sent: Thursday, June 10, 2010 12:27 PM
To: Kim Merriam; laurie <@t> conxis.com
Cc: Histonet
Subject: RE: [Histonet] Histodeck Flash Cards

Where did you purchase them?

Lynn Burton
Lab Assoc. I
Animal Disease Lab
Galesburg, Il 61401

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Kim Merriam
Sent: Thu 6/10/2010 7:57 AM
To: laurie <@t> conxis.com
Cc: Histonet
Subject: Re: [Histonet] Histodeck Flash Cards

Hi Laurie,

I purchased them because I am thinking about taking the HTL in the next
year or so.  The are pretty nice, they are divided up into categories
(fixation, processing, etc) and each category has a different color edge
on the card.  They have questions on the front with the answers and
explanations on the back.  They were pretty expensive (75-80, I think),
but would probably help out a lot when studying for an exam (a
non-histologist could ask the questions and help you study)!  I will
probably enlist my 9 year old, who gets a kick out of this kind of
thing.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

________________________________
From: "laurie <@t> conxis.com" <laurie <@t> conxis.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tue, June 8, 2010 11:20:46 AM
Subject: [Histonet] Histodeck Flash Cards

Hi Everyone,

I was wondering if anyone out there has used the Histodeck flash cards
to study for the HTL exam?  Just curious.

Thanks,
Laurie

***********************

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------------------------------

Message: 2
Date: Thu, 10 Jun 2010 10:48:41 -0700
From: Cindy DuBois <integrated.histo <@t> gmail.com>
Subject: [Histonet] Thin Prep
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTinjUhktzj2-B77loPCNbjQLDPUD-y_Eb5o_hdtq <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Our lab is currently looking for another Thin Prep machine.  Does
anyone know where I can locate a used model?

Cindy DuBois
Integrated Pathology
Stockton, CA

------------------------------

Message: 3
Date: Thu, 10 Jun 2010 15:56:30 -0400
From: rgrow <@t> bmnet.com
Subject: [Histonet] Full Time HT in E. Tennessee
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OF517E3FD9.46AFC390-ON8525773E.006A8142-8525773E.006D8B13 <@t> bmnet.com>
Content-Type: text/plain; charset=UTF-8

Blount Memorial Hospital in Maryville TN has a full time histology
technician position open Monday thru Friday.  We have good benefits, with
possible relocation assistance for the right candidate. If you are
interested in working for a hospital that cares about its patients, we may
be what you are looking for.
We have a new (3yrs) histology laboratory, excellent ventilation, and ample
workspace. We do approximately 12,000 cases/yr routine small and large
surgicalâ€™s, & biopsies with frozen, routine H&E, special stains, and about
33 IHC antibodies (automated). The processing room is separate with 3
tissue processors,  2 run nightly, with one for same-day turn-around cases.
General histology experience with certification preferred. Some
Immunohistochemistry would be helpful.  Weekdays 7-3:30pm with occasional
variance in schedule as needed.  Must demonstrate competency and
successfully complete the on-the-job orientation through the histology
section of the laboratory. Perform all duties of a Histology Technician and
other duties as assigned.
Applicants must meet the educational and training requirements necessary
for certification by the American Society of Clinical Pathology as a
Histology Technician or have experience equal to certification.

We are located just 20 minutes from the beautiful Smoky Mountain National
Park and experience 4 wonderful seasons!  All types of outdoor activities
are possible.  Maryville is host to the annual Foothills Fall Festival with
top name entertainment and crafts, and just 30 minutes from Knoxville's
cultural events and entertainment, as well as UT football, basketball, etc.
Gatlinburg and Pigeon Forge are close too!

Anyone interested please visit our website at blountmemorial.org. to fill
out an application and attach a resume.

Thanks,
Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN  37804-5016
(865) 977-4744
(865) 977-5766 Fax
________________________________

This communication may contain protected health information (PHI) that is
legally protected from inappropriate disclosure by the Privacy Standards of
the Health Insurance Portability and Accountability Act (HIPAA) and
relevant Tennessee Laws. If you are not the intended recipient, please note
that any  dissemination, distribution or copying of this communication is
strictly prohibited. If you have received this message in error, you should
notify the sender immediately by telephone or by return e-mail and delete
this message from your computer. Direct questions to the Blount Memorial
Hospital Privacy Officer at 865-977-4675.

------------------------------

Message: 4
Date: Thu, 10 Jun 2010 15:05:04 -0700 (PDT)
From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
Subject: Re: [Histonet] Ocular fiative
To: tahseen <@t> brain.net.pk, histonet <@t> lists.utsouthwestern.edu
Message-ID: <179259.91336.qm <@t> web114418.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Tahseen-

I work in the world of animal/research histology.  I have spent many years working with rodent and nonrodent eyes, developing my skills and knowledge. 

Different fixatives work well with different parts of the eye.  10% NBF might wotk well for cornea, bulbar and palpebral conjunctiva.  But it is just about the worst fixative for retina.  Most glutaraldehyde fixatives are hypertonic and cause the eyes to collaspe unless "windows" (~2-5 mm holes cut along lateral or medial margins of the eye to allow fixative to penetrate the vitreous humour) are cut in the eyes ~1-5 hours after they go into fix.  But glut is very good for most eye structures, although it tends to overharden the lens.  The very best retina fixation I have ever seen is with (real) Zenker's fixative.  The Zn Zenker substitutes I have seen are terrible for this application.

For most eye structures, Davidson's can work well.  Overfixing (more than 24 hours) can cause tissue swelling and overhardening.  But in a hospital setting, this may be just what would be needed.  There is more than one recipe for Davidson's I've seen over the years, some having been altered to better fix testes.  Check with the books to make sure you have the original recipe.

It will be interesting to hear what our hospital colleagues use for eyes.  I would guess most use 10% NBF.  And I am sure their pathologists say that this is just fine.

Good luck!

Joe Saby, BA HT

________________________________
From: "tahseen <@t> brain.net.pk" <tahseen <@t> brain.net.pk>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thu, June 10, 2010 12:53:22 AM
Subject: [Histonet] Ocular fiative

HI all,
We are using Alcoholic formalin fixative for ocular (Eye) specimens,
Reference Tech Sample (ASCP) HT-2(1995), since last 15 year without facing
any problem.
Now one of our pathologists (New joiner) wants to fix Ocular in 10%NBF.
I would lick to know how are doing others, and is there any comparison
between 10%NBF verses Alcoholic fixative for Ocular (Eye).
Thanks,
Muhammad Tahseen
Histology Supervisor
SKMCH&RC Lahore
Pakistan

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------------------------------

Message: 5
Date: Fri, 11 Jun 2010 11:18:31 -0400
From: "Kaye Ryan" <kryan <@t> nfderm.com>
Subject: [Histonet] Job Opening 
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<DCB07C3EF84D92439E5A453D8D5B5EB028CBCC <@t> exchserver.NFDA.com>
Content-Type: text/plain;	charset="us-ascii"

Hi Everyone,

We are a derm lab located in beautiful Jacksonville, Florida.  We are
looking for a full time histologist with at least one year experience.
Must be ASCP certified and/or have a current Florida technologist
license.  Shift will be Monday - Friday, 6:00 am - 2:30 p.m.  Great
working atmosphere with a wonderful small team.  Duties to include
routine histology, IHC, and an occasional special stain.  Competitive
salary and good benefits.  If interested, please contact me by e-mail or
Gloria Thompson (HR) at 904-398-0547, ext 1106 for more information.

Have a great day,

Kaye Ryan

Histology Supervisor

------------------------------

Message: 6
Date: Fri, 11 Jun 2010 08:45:14 -0700
From: jessamine winer <jpwiner <@t> gmail.com>
Subject: [Histonet] Can you do Masson's trichrome on frozen sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTim7uJU3Rd5xixB5ZnipWkBwpnah_s-5_9e_oxuM <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi,

We normally do trichrome on paraffin sections but I was wondering if it's
possible to do on frozen sections.  I don't want to waste my time if it is
just going destroy the tissue.  If it will work what thickness sections do I
need, what fixative and are there any modifications I need to make to the
standard protocol.

Thanks,
Jessamine

------------------------------

Message: 7
Date: Fri, 11 Jun 2010 09:53:58 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW:  [Histonet] ink on film coverslips
To: "'Amy Farnan'" <Farnana <@t> nehealth.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <EC14FB1FFDD14F708ADAF5F981BA730C <@t> prueggihctechlt>
Content-Type: text/plain;	charset="us-ascii"

Try acetone which will also remove the tape cs, maybe that is what you
should do, remove the tape coverslip in acetone and recover it.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Farnan
Sent: Thursday, June 10, 2010 5:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] ink on film coverslips

Hi everyone,
I am trying to completely remove the dotting ink from our slides. I have
tried alcohol and it takes off the blue dotting ink but the green ink leaves
a yellow faded dot on the cover slip. The cover slips that we use are the
film coverslip so I think the ink gets absorbed somehow. Do you have any
ideas that may take the ink off of the cover slips? 
I would like to avoid taking the coverslip off the slide if possible.

Thank you,
Amy Farnan
Northeast Health

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------------------------------

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End of Histonet Digest, Vol 79, Issue 11
****************************************