[Histonet] AW: INI-1

[Kappeler, Andreas (PATHOLOGY)]

Hi Hazel

We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100 mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5. Incubation at RT, 60 min. Visualization with polymer-based HRP system/DAB. Hope this helps.

Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

P.S. when asking about advice on antibodies, try to be as specific as possible: clone, Ig-conc and/or dilution, retrieval protocols tried, visualization systems tried. Helps o narrow down possilbe problems.

________________________________________
Von: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] im Auftrag von Horn, Hazel V [HornHV <@t> archildrens.org]
Gesendet: Freitag, 4. Juni 2010 13:55
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] INI-1

We are having problems with this immunostain.  If your lab is
successfully using this antibody will you please help me out?

Where do you purchase your antibody?  Is it RTU?  What type of antigen
retrieval?  Incubation time?

Thanks.



Hazel Horn

Hazel Horn, HT/HTL (ASCP)

Supervisor of Histology

Arkansas Children's Hospital

1 Children's Way    Slot 820

Little Rock, AR   72202



phone   501.364.4240

fax        501.364.3155



visit us on the web at:    www.archildrens.org



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