[Histonet] Reticulum stain wash-offs

Cheryl Crowder ccrowder <@t> vetmed.lsu.edu
Thu Jul 29 23:51:48 CDT 2010

Rather than use Halt (or other adhesive) in your water bath, have you tried 
using charged slides - those used for IHC.  Then air-dry or heat dry the 
tissues before deparaffinizing.   Any water left in the tissue will cause 
wash-offs.  Do not lower the pH of the silver solution.

Cheryl Crowder, BA, HTL(ASCP)

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