[Histonet] : Gulf oil ? Histonet Digest, Vol 80, Issue 34
pvlies <@t> yahoo.com
pvlies <@t> yahoo.com
Thu Jul 29 12:42:03 CDT 2010
Yes, ORO stains lipids but I don't think it would work for crude. Would you look for artifact and tissue reaction?
Pam Vlies, HT-ASCP
Waukegan IL
Sent on the Sprint® Now Network from my BlackBerry®
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Today's Topics:
1. Gordon and Sweets retic stain (Johnson, Nacaela)
2. RE: Histonet Digest, Vol 80, Issue 33 (Joanne Clark)
3. Sakura VIP6 update (Cynthia Pyse)
4. dissection aide (Tench, Bill)
5. Refrigerate formalin? (Breeden, Sara)
6. Re: Refrigerate formalin? (Rene J Buesa)
7. REFRIG FORMALIN (Tench, Bill)
8. Re: Refrigerate formalin? (Mark Ray)
9. Re: ? on Gulf oil spill (Lesley Weston)
10. PECAM-1 (sc-1506R) staining (Victor Wong)
11. RE: Refrigerate formalin? (Johnson, Nacaela)
12. NEW Opportunity with a World Leader in IHC in New England!
(Matthew Ward)
13. Re: Refrigerate formalin? (Geoff McAuliffe)
14. FAST interpretation (sarah Tabatabaei)
15. Cool Formalin (Breeden, Sara)
16. plastics (Dixon,Maryann)
17. knife holder (Dixon,Maryann)
18. p53 IHC staining in mouse (Ross Benik)
----------------------------------------------------------------------
Message: 1
Date: Wed, 28 Jul 2010 12:11:42 -0500
From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
Subject: [Histonet] Gordon and Sweets retic stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6DBD71C31D7E444482E5D3DFBC202D260245D232 <@t> txhous1eb012.uson.usoncology.int>
Content-Type: text/plain; charset="us-ascii"
Hello!
I am having a problem with my tissue washing off of the slides during
the retic stain. The majority of the tissue is falling off during the
Working silver solution step because of the alkalinity of the solution.
The tissue is bone marrow and I use Halt in my water bath. My thoughts
are to add a mild acid to the working solution to bring the pH down, but
I am not sure of what the effects are on the solution. I do not want the
silver to not impregnate. Has anyone had this problem before and found a
solution?
Thanks,
Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
Email: Nacaela.Johnson <@t> USOncology.com
</pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre>
------------------------------
Message: 2
Date: Wed, 28 Jul 2010 12:34:49 -0600
From: "Joanne Clark" <jclark <@t> pcnm.com>
Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 33
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39C012F797B <@t> mail.pcnm.com>
Content-Type: text/plain; charset="iso-8859-1"
We use a similar product called Dissect Aid, from Decal Corp. IHC staining has never been an issue; we never run IHC on the lymph nodes from colon cancer cases. We usually do the IHC on the tumor itself which is fixed in formalin. This kind of product is very useful in helping to find all those pesky little nodes that like to hide in the fat by turning them white. Cancer protocols require that at least 13 nodes be submitted. As histo supervisor, I also do the grossing in my facility, so I understand why your PA is interested in this kind of product (it can be difficult to come up with this magic number of 13 without it). However, if you do run IHC on the nodes from these cases, you would indeed have to revalidate your markers with this as your primary fixative or as a post fixative.
Joanne Clark, HT, MLT
Histology Supervisor
Pathology Consultants of New Mexico
Roswell, NM
-----------------------------
Message: 2
Date: Tue, 27 Jul 2010 15:03:33 -0300
From: "Hayes, Randi (HorizonNB)" <Randi.Hayes <@t> horizonnb.ca>
Subject: [Histonet] Using GEWF solution and IHC staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C2889F00E87E8D4EA5798737D8F7F362A73680 <@t> RHAEX1.RHA-RRS.CA>
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At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how "safe" it is to use when staining for IHC. Does anyone have much experience with this or know of a study (studies) that have been done to verify that the ethanol is not destroying antigen sites? We're a little concerned......
Randi Hayes, MLT
Histology Supervisor / Superviseur d'Histologie
Horizon Health Network / Réseau de santé Horizon
(506) 860-2157
randi.hayes <@t> HorizonNB.ca <http://www.me.com/mail/>
www.HorizonNB.ca <http://www.horizonnb.ca/>
------------------------------
Message: 3
Date: Wed, 28 Jul 2010 14:40:21 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: [Histonet] Sakura VIP6 update
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <002101cb2e84$55ced450$016c7cf0$@com>
Content-Type: text/plain; charset="us-ascii"
Thanks everyone for your responses. Sakura has scheduled an upgrade of my
rotary and gate valve. I hope this is the answer I need.
Cindy
------------------------------
Message: 4
Date: Wed, 28 Jul 2010 12:12:30 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] dissection aide
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A54A9 <@t> MAIL1.pph.local>
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I don't see much advantage of this over plain old Carnoy's fixative,
other than missing the wiff of choroform. Ethanol should not be a
problem with IHC, but as you said, revalidation is required. The magic
number of nodes is 12.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
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Message: 5
Date: Wed, 28 Jul 2010 14:41:04 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Refrigerate formalin?
To: <histonet <@t> lists.utsouthwestern.edu>
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We're getting ready to move into our new building (YAHOO!) in early
September - finally. In planning where we will store our cases for the
required 6-week retention time, I have proposed that the tissues (in 10%
NBF) be shelved in the walk-in cooler (4 degrees C). Is there any
reason tissues-in-formalin could NOT be stored refrigerated for that
length of time? It is extremely rare that we are required to pull and
recut wet tissue, but that possibility always exists. Thanks, everyone.
Sally Breeden, HT(ASCP)
Veterinary Diagnostic Services
New Mexico Department of Agriculture
700 Camino de Salud NE
Albuquerque, NM 87108
505-841-2576
------------------------------
Message: 6
Date: Wed, 28 Jul 2010 13:49:52 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Refrigerate formalin?
To: histonet <@t> lists.utsouthwestern.edu, SaraBreeden
<sbreeden <@t> nmda.nmsu.edu>
Message-ID: <304094.35306.qm <@t> web65715.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
There is nothing against cooling formalin fixed tissues, but my question is WHY?
I see it as a waste of refrigerated space.
On the other hand also, IF by any change there is a formalin spill inside the walk-in cooler room, that will be a royal mess to decontaminate.
René J.
--- On Wed, 7/28/10, Breeden, Sara <sbreeden <@t> nmda.nmsu.edu> wrote:
From: Breeden, Sara <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Refrigerate formalin?
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, July 28, 2010, 4:41 PM
We're getting ready to move into our new building (YAHOO!) in early
September - finally. In planning where we will store our cases for the
required 6-week retention time, I have proposed that the tissues (in 10%
NBF) be shelved in the walk-in cooler (4 degrees C). Is there any
reason tissues-in-formalin could NOT be stored refrigerated for that
length of time? It is extremely rare that we are required to pull and
recut wet tissue, but that possibility always exists. Thanks, everyone.
Sally Breeden, HT(ASCP)
Veterinary Diagnostic Services
New Mexico Department of Agriculture
700 Camino de Salud NE
Albuquerque, NM 87108
505-841-2576
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Wed, 28 Jul 2010 13:50:28 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] REFRIG FORMALIN
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A54AA <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii
You must have a heck of a lot of space in the frig. I am envious. I
don't think refrigerating your specimens would create any problems.
Everyone i know stores them in some inconvenient corner of a morgue at
room temperature.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
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Message: 8
Date: Wed, 28 Jul 2010 16:24:37 -0500
From: Mark Ray <darkdaym <@t> comcast.net>
Subject: Re: [Histonet] Refrigerate formalin?
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4C50A015.40005 <@t> comcast.net>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
It is usually recommended that 10% Formalin Fixative be stored at
temperatures above about 10C to minimize polymerization of
Formaldehyde. You might confirm this with the manufacturer of your
Formalin Fixative. The label may also say something about this.
Breeden, Sara wrote:
> We're getting ready to move into our new building (YAHOO!) in early
> September - finally. In planning where we will store our cases for the
> required 6-week retention time, I have proposed that the tissues (in 10%
> NBF) be shelved in the walk-in cooler (4 degrees C). Is there any
> reason tissues-in-formalin could NOT be stored refrigerated for that
> length of time? It is extremely rare that we are required to pull and
> recut wet tissue, but that possibility always exists. Thanks, everyone.
>
>
>
> Sally Breeden, HT(ASCP)
>
> Veterinary Diagnostic Services
>
> New Mexico Department of Agriculture
>
> 700 Camino de Salud NE
>
> Albuquerque, NM 87108
>
> 505-841-2576
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 9
Date: Wed, 28 Jul 2010 15:54:18 -0700
From: Lesley Weston <leswes <@t> shaw.ca>
Subject: Re: [Histonet] ? on Gulf oil spill
To: jstaruk <jstaruk <@t> masshistology.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <378EA25A-D24E-4961-9684-6370B09BC5A3 <@t> shaw.ca>
Content-Type: text/plain; charset=us-ascii
Oil Red O might work, since it stains lipids.
Lesley Weston.
On 2010-07-28, at 7:26 AM, jstaruk wrote:
> Hi all,
>
> I have a customer who wants to study the amounts of oil accumulation in the
> gills of fish from the Gulf of Mexico. Does anyone have any suggestions as
> to what techniques might stain crude oil?
>
> Thanks
>
> Jim
>
> _______________________
> James E. Staruk HT(ASCP)
> www.masshistology.com
> www.nehorselabs.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Wed, 28 Jul 2010 21:05:45 -0700 (PDT)
From: Victor Wong <vhlwong <@t> yahoo.com>
Subject: [Histonet] PECAM-1 (sc-1506R) staining
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <983283.48384.qm <@t> web52701.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear all,
Thank you for all the professional suggestion of the previous IHC staining and finally I got some positive results.
Then comes problem on the PECAM-1 IHC. I am using the Santa Cruz sc-1506R (it is a anti-RAT antibody from RABBIT). I stain decalcified rat spine paraffin sections, with following steps:
antigen retrieval (~95C MW, DAKO AR solution pH6 for 20 minutes);
DAKO's Dual Enzyme block for 10 minutes;
3% normal goat serum for 20 minutes;
1:50 primary antibody at 4C for overnight;
DAKO's Envision anti-rabbit kit for 30 minutes;
DAKO's DAB solution to develop
I used rat liver and kidney as positive controls. I got background staining on hepatocytes but none staining on vessels. On kidney section, nucleus and lining within tubules and basement membrane of glomerulus were also stained but not that bad as liver. I cannot say it was totally non-specific as some nucleus were not stained.
I think PECAM-1 IHC needs more specific conditons. Could any histoneters have any suggestion?
Many thanks in advance.
Victor
------------------------------
Message: 11
Date: Thu, 29 Jul 2010 07:49:02 -0500
From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
Subject: RE: [Histonet] Refrigerate formalin?
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6DBD71C31D7E444482E5D3DFBC202D260245D237 <@t> txhous1eb012.uson.usoncology.int>
Content-Type: text/plain; charset="us-ascii"
I had a retic kit that was put in the refrigerator by one of my
collegues. The formalin was stated to be stored at room temp, but was
left in the box. The cold temperature broke the formalin down and it no
longer worked to reduce the silver. My suggestion would be to keep them
at room temperature to be on the safe side.
Thanks,
Nacaela Johnson
Histology Technician
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
Email: Nacaela.Johnson <@t> USOncology.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Wednesday, July 28, 2010 3:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Refrigerate formalin?
We're getting ready to move into our new building (YAHOO!) in early
September - finally. In planning where we will store our cases for the
required 6-week retention time, I have proposed that the tissues (in 10%
NBF) be shelved in the walk-in cooler (4 degrees C). Is there any
reason tissues-in-formalin could NOT be stored refrigerated for that
length of time? It is extremely rare that we are required to pull and
recut wet tissue, but that possibility always exists. Thanks, everyone.
Sally Breeden, HT(ASCP)
Veterinary Diagnostic Services
New Mexico Department of Agriculture
700 Camino de Salud NE
Albuquerque, NM 87108
505-841-2576
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
</pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre>
------------------------------
Message: 12
Date: Thu, 29 Jul 2010 09:15:03 -0400
From: "Matthew Ward" <MW <@t> PersonifySearch.com>
Subject: [Histonet] NEW Opportunity with a World Leader in IHC in New
England!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <010f01cb2f20$0eb960c0$2c2c2240$@com>
Content-Type: text/plain; charset="us-ascii"
Good Morning All,
Our team at Personify is currently partnered with a World Leader in IHC that
is currently looking for a Field Support Specialist in the New England area.
This is the perfect opportunity for a histotech to move off the bench and
get into the field on the manufacturers side!
The position offers the following:
Base Salary!
Bonus!
Car Allowance and Paid Expenses!
Outstanding Benefits!
Opportunity for advancement!
Please contact me immediately to learn more!
Matt Ward
Account Executive
Personify
201 Shannon Oaks Circle, Suite 101
Cary, North Carolina 27511
(Tel) 800.875.6188 direct ext 103
(Fax) 919.460.0642
<http://www.personifysearch.com/> www.personifysearch.com
------------------------------
Message: 13
Date: Thu, 29 Jul 2010 09:47:59 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Refrigerate formalin?
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4C51868F.90302 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I agree with Rene and Mark.
Geoff
Breeden, Sara wrote:
> We're getting ready to move into our new building (YAHOO!) in early
> September - finally. In planning where we will store our cases for the
> required 6-week retention time, I have proposed that the tissues (in 10%
> NBF) be shelved in the walk-in cooler (4 degrees C). Is there any
> reason tissues-in-formalin could NOT be stored refrigerated for that
> length of time? It is extremely rare that we are required to pull and
> recut wet tissue, but that possibility always exists. Thanks, everyone.
>
>
>
> Sally Breeden, HT(ASCP)
>
> Veterinary Diagnostic Services
>
> New Mexico Department of Agriculture
>
> 700 Camino de Salud NE
>
> Albuquerque, NM 87108
>
> 505-841-2576
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 14
Date: Thu, 29 Jul 2010 07:04:29 -0700 (PDT)
From: sarah Tabatabaei <sarah_taba <@t> yahoo.com>
Subject: [Histonet] FAST interpretation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <395564.60128.qm <@t> web45707.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi,
I'm using FAST profile (Fast green, Alcian Blue, Safranin-O, and Tartrazine) to stain my human Intervertebral Discs. how can I figure out which color resembles which type of tissue?
Regards
-Sarah
------------------------------
Message: 15
Date: Thu, 29 Jul 2010 09:04:46 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Cool Formalin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B02E47294 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Thanks to everyone that replied to my question about refrigerating 10%
NBF for up to 6 weeks. I'll fold your information into the decision.
Sally Breeden, HT(ASCP)
Veterinary Diagnostic Services
New Mexico Department of Agriculture
700 Camino de Salud NE
Albuquerque, NM 87108
505-841-2576
------------------------------
Message: 16
Date: Thu, 29 Jul 2010 11:34:43 -0400
From: "Dixon,Maryann" <dixonm <@t> ufl.edu>
Subject: [Histonet] plastics
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FA9822F11A2EDB4EABEB80DFA3534B970555F961 <@t> HSC-CMS01.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Hi histoland,
Thinking of getting into plastics and need to know information, pros/cons about them. I've never worked with anything else except paraffin. I will be embedding mostly osteosarcomas. Can someone please give me a call or email me. All suggestions are very appreciated. Thank you.
MaryAnn Dixon BS, HT (ASCP)cm
Biological Scientist
Surgical Oncology
UF College of Veterinary Medicine
Phone (352) 294-4516
Email: dixonm <@t> ufl.edu
------------------------------
Message: 17
Date: Thu, 29 Jul 2010 12:54:51 -0400
From: "Dixon,Maryann" <dixonm <@t> ufl.edu>
Subject: [Histonet] knife holder
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FA9822F11A2EDB4EABEB80DFA3534B970555F962 <@t> HSC-CMS01.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Greetings histonetters!!!
Does anyone out there have a paraffin knife holder (knife holder B) for a Leica SM2500 polycut microtome that you might like to part with. I am in need of finding one. Thank you.
MaryAnn Dixon BS, HT (ASCP)cm
Biological Scientist
Surgical Oncology
UF College of Veterinary Medicine
Phone (352) 294-4516
Email: dixonm <@t> ufl.edu
------------------------------
Message: 18
Date: Thu, 29 Jul 2010 10:56:56 -0600
From: "Ross Benik" <ross <@t> premierlab.com>
Subject: [Histonet] p53 IHC staining in mouse
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B0A8EDA <@t> server.PremierLab.local>
Content-Type: text/plain; charset="us-ascii"
Hi everyone,
I am trying to accomplish IHC staining for a rabbit anti p53 antibody in
mouse tissue however all I am getting is background and IgG staining. I
run my human positive control tissue along with the same protocol
parameters and the staining is perfect. I have tried two abcam
antibodies (ab32049 and ab4060) and both are not working correctly on
mouse tissue. Does anyone know of a p53 antibody that is known to work
in mouse tissue?
Thanks,
Ross
------------------------------
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