[Histonet] p53 IHC staining in mouse

[Ross Benik]

Hi everyone, 

 

I am trying to accomplish IHC staining for a rabbit anti p53 antibody in
mouse tissue however all I am getting is background and IgG staining.  I
run my human positive control tissue along with the same protocol
parameters and the staining is perfect.  I have tried two abcam
antibodies (ab32049 and ab4060) and both are not working correctly on
mouse tissue.  Does anyone know of a p53 antibody that is known to work
in mouse tissue? 

 

Thanks, 

Ross