[Histonet] Using GEWF solution and IHC staining

Mark Tarango marktarango <@t> gmail.com
Tue Jul 27 16:58:25 CDT 2010

Ethanol/alcohol is what will process the specimen.  If the tissue is fixed
would it really matter that the tissue came into contact with ethanol?

On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) <
Randi.Hayes <@t> horizonnb.ca> wrote:

> At a recent conference, our PA learned of using GEWF (glacial acetic acid,
> ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph
> Node retrieval in Colorectal Cancer resections.  Although a good idea, I'm
> wondering how "safe" it is to use when staining for IHC.  Does anyone have
> much experience with this or know of a study (studies) that have been done
> to verify that the ethanol is not destroying antigen sites?  We're a little
> concerned......
> Randi Hayes, MLT
> Histology Supervisor / Superviseur d'Histologie
> Horizon Health Network / Réseau de santé Horizon
> (506) 860-2157
> randi.hayes <@t> HorizonNB.ca <http://www.me.com/mail/>
> www.HorizonNB.ca <http://www.horizonnb.ca/> <http://www.horizonnb.ca/>
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