[Histonet] Using GEWF solution and IHC staining

[Patrick Laurie]

Sounds like to me that it might involve another revalidation of each one of
your antibodies.

Good luck

On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) <
Randi.Hayes <@t> horizonnb.ca> wrote:

> At a recent conference, our PA learned of using GEWF (glacial acetic acid,
> ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph
> Node retrieval in Colorectal Cancer resections.  Although a good idea, I'm
> wondering how "safe" it is to use when staining for IHC.  Does anyone have
> much experience with this or know of a study (studies) that have been done
> to verify that the ethanol is not destroying antigen sites?  We're a little
> concerned......
>
>
> Randi Hayes, MLT
> Histology Supervisor / Superviseur d'Histologie
> Horizon Health Network / Réseau de santé Horizon
> (506) 860-2157
> randi.hayes <@t> HorizonNB.ca <http://www.me.com/mail/>
> www.HorizonNB.ca <http://www.horizonnb.ca/> <http://www.horizonnb.ca/>
>
>
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com