[Histonet] artifacts in sections

faith14913 <@t> aol.com faith14913 <@t> aol.com
Fri Jul 23 19:21:40 CDT 2010


I have always soaked my biopsies on ice water.  It always seems to help.

Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Fri, 23 Jul 2010 13:01:46 
To: <histonet <@t> lists.utsouthwestern.edu>
Reply-To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 80, Issue 27

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Today's Topics:

   1. Laboratory Labeling Systems... (Barone, Carol )
   2. Re: Histonet Digest, Vol 80, Issue 26 (Stephanie Rodriguez)
   3. Histology metrics (Scherer, Kris)
   4. RE: HPV testing (McMahon, Loralee A)
   5. RE: Biocare P63 with Dako Flex detection (Cynthia Pyse)
   6. biohazard bags (Jeff Birkner)
   7. RE: biohazard bags (Stacy McLaughlin)
   8. Re: biohazard bags (DKBoyd <@t> chs.net)
   9. Sectioning problem (kgrobert <@t> rci.rutgers.edu)
  10. Re: Sectioning problem (Bryan Llewellyn)
  11. Staining dish for vertical 30-slide rack (Morken, Tim)
  12. RE: biohazard bags (Rathborne, Toni)
  13. p-TBK-1 Ab (Michelle Mathews)
  14. Artifacts in histology section (Aazath Raj)
  15. Microwave Decalcification (Figliuolo, Leticia)
  16. Re: Artifacts in histology section (Rene J Buesa)
  17. artifacts of sectioning (Tench, Bill)
  18. Formalin substitutes (Weaver, Colin)
  19. RE: artifacts of sectioning (Matt Brooks)
  20. HPV (Rick.Garnhart <@t> memorialhealthsystem.com)


----------------------------------------------------------------------

Message: 1
Date: Thu, 22 Jul 2010 13:21:26 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] Laboratory Labeling Systems...
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <37E4BAC017F57141AF64FAA5AEB04CE8033A7E87 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain;   charset="us-ascii"

Histonetters....What are your thoughts: 
 
We are getting ready to purchase a labeling system for our Core
Facility. We have limited $'s and have demo'ed three systems. We have
many different projects,with varying numbers of samples, blocks and
slides. We would like to cover the widest amount of needs, with the
system....not just cassettes and slides, but samples in freezers to
antibodies we have alloquotted. We are about to purchase a two-D
bar-coding system that can label everything we need and work with our
present EXCEL data bases.  We are leaning toward a Brady System, where
we can purchase individually the parts we need for labeling cassettes to
eppy's and we especially like that they... not only have a label that
can be attached to frozen vials... but that there are several types of
scanners available for the different stations in our lab. This system
meets our CER requirements and is up-gradable for adding more stations
if needed at alater time. 
 
Sounds too good to be true....so that is where you all come in. 
 
Is anyone using a Brady Labeling System? Good? Bad? Problems? What are
your thoughts?  Thanks for the impromptu survey. We plan to order in
August, if there are no "big" issues, with those that use it. We demo's
the labels and are happy with them. We are going alpha numeric/2-D...3
lines of text, is needed for our research work. Thanks for your
thoughts. 
 
C. Barone
Director Histotechnology Core Lab
cbarone <@t> nemours.org
 


------------------------------

Message: 2
Date: Thu, 22 Jul 2010 10:44:10 -0700
From: Stephanie Rodriguez <srodriguez <@t> phenopath.com>
Subject: [Histonet] Re: Histonet Digest, Vol 80, Issue 26
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C86DD17A.D08%srodriguez <@t> phenopath.com>
Content-Type: text/plain;   charset="US-ASCII"

As do we.


Stephanie Rodriguez, HTL(ASCP), QIHC
Senior Molecular Technologist-FISH
IHC Technologist III
Phenopath Laboratories
Seattle, WA
(206) 374-9000 




On 7/22/10 10:04 AM, "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu> wrote:

> 
> Message: 4
> Date: Thu, 22 Jul 2010 08:18:27 -0700
> From: "Tench, Bill" <Bill.Tench <@t> pph.org>
> Subject: [Histonet] hpv testing on head and neck ca's
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5480 <@t> MAIL1.pph.local>
> Content-Type: text/plain; charset=us-ascii
> 
> We do p16 on squamous ca of the head and neck region routinely
>  
> Bill Tench
> Associate Dir. Laboratory Services
> Chief, Cytology Services
> Palomar Medical Center
> 555 E. Valley Parkway
> Escondido, California  92025
> Bill.Tench <@t> pph.org
> Voice: 760- 739-3037
> Fax: 760-739-2604



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intended recipients and may contain privileged information. Any unauthorized 
review, use, disclosure or distribution is prohibited. If you are not the intended 
recipient, please contact the sender by e-mail and destroy all copies of the 
original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. 
at (206) 374-9000.


------------------------------

Message: 3
Date: Thu, 22 Jul 2010 13:43:52 -0400
From: "Scherer, Kris" <kris <@t> idxpathology.com>
Subject: [Histonet] Histology metrics
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <0C15E5FC826B334BA019E489846D02357E06C23C55 <@t> RTWECS03.lca.labcorp.com>
Content-Type: text/plain; charset="us-ascii"

We are in need of a metrics for use in requesting an additional histotech.  Does anyone have a system in place for presenting the need for an additional FTE, based on volume, worklow, vacations, etc?
----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer <@t> labcorp.com or call (877) 23-HIPAA / (877) 234-4722. 




------------------------------

Message: 4
Date: Thu, 22 Jul 2010 13:47:54 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] HPV testing
To: Richard Cartun <Rcartun <@t> harthosp.org>, Histonet
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <C27AA2A01CEF31469813089E226F582E02D03DAE1C <@t> URMCMS7.urmc-sh.rochester.edu>
    
Content-Type: text/plain; charset="us-ascii"

Hi Dr. Cartun, 

We are running the HPV Genepoint test from Dako (in situ test).  Along with the EGFR IHC.  


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard Cartun [Rcartun <@t> harthosp.org]
Sent: Thursday, July 22, 2010 11:01 AM
To: Histonet
Subject: [Histonet] HPV testing

What are labs doing for HPV testing on head and neck cancers when a request is received from a clinician?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 5
Date: Thu, 22 Jul 2010 15:10:24 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] Biocare P63 with Dako Flex detection
To: <Cathy.Crumpton <@t> tuality.org>,   <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cb29d1$89f157b0$9dd40710$@com>
Content-Type: text/plain;   charset="us-ascii"

Cathy
Have you tried adding an EnVision FLEX mouse linker to your protocol.
Sometimes that will work.

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Cathy.Crumpton <@t> tuality.org
Sent: Wednesday, July 21, 2010 3:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biocare P63 with Dako Flex detection


   Hi  all, I have ran into a troubleshooting dilemma and was wonderi=g
   if  anyone  else could help.  Is there anyone out there that uses the
Biocare  Medical  P63 concentrated antibody with the Dako TRS and Flex
   detec=tion  kit?  I am curious what dilution you use and if you have
   to  do  an=ything  special  for  the  P63 (extra long retrieval, DAB
   enhancer, etc). = am having problems getting a strong signal for the
   basal cells in prosta=e.  I am running it at 1:50 with 40 minutes in
   pH  9  retrieval  (which  =seems  too  long to me but does help).  A
   dilution with the same protoco= at 1:100 is way too light.

   Thanks,
    <=DIV>
   Cathy Crumpton HT(ASCP), Histology Lead
   Tuality Community Hosp=tal
   Hillsboro, OR 97123
   (503)681-1292
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------------------------------

Message: 6
Date: Thu, 22 Jul 2010 14:11:18 -0500
From: "Jeff Birkner" <jbirkner <@t> colabserv.com>
Subject: [Histonet] biohazard bags
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <EFD452EEB5F9A04D8919C4228C5C392D04DC6D4F <@t> p1ws0001.orhcnet.local>
Content-Type: text/plain;   charset="us-ascii"

We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner <@t> colabserv.com

 


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Message: 7
Date: Thu, 22 Jul 2010 15:38:01 -0400
From: "Stacy McLaughlin" <Stacy_McLaughlin <@t> cooley-dickinson.org>
Subject: RE: [Histonet] biohazard bags
To: "Jeff Birkner" <jbirkner <@t> colabserv.com>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <CB8866A9D4ECB049968DB571CF4863A3060DE0DF <@t> cdhmail01.cooley-dickinson.org>
    
Content-Type: text/plain;   charset="iso-8859-1"

Re-use of specimen transport bio-bags is a big NO-NO in my laboratory.  You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) 
You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean.  You don't know the practices of the person that collected it.  This could be a big liability and the possible consequences just aren't worth it.  
Just my 2 cents.
Stacy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jeff
Birkner
Sent: Thursday, July 22, 2010 3:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] biohazard bags


We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner <@t> colabserv.com

 


The information contained in this communication may be confidential, is intended
only for the use of the recipient(s) named above, and may be legally privileged.
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution, or copying of this communication,
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communication in error, please return it to the sender immediately and delete the
original message and any copy of it from your computer system.  If you have any
questions concerning this message, please contact the sender. 
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 8
Date: Thu, 22 Jul 2010 15:42:34 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] biohazard bags
To: "Jeff Birkner" <jbirkner <@t> colabserv.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
    histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
    <OF57D1F229.06C9A70C-ON85257768.006C1721-85257768.006C44B7 <@t> chs.net>
Content-Type: text/plain;   charset="US-ASCII"

We were doing just that and our Infection Control Nurse has banded this 
practice.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







"Jeff Birkner" <jbirkner <@t> colabserv.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/22/2010 03:11 PM

To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] biohazard bags






We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner <@t> colabserv.com

 


The information contained in this communication may be confidential, is 
intended
only for the use of the recipient(s) named above, and may be legally 
privileged.
If the reader of this message is not the intended recipient, you are 
hereby 
notified that any dissemination, distribution, or copying of this 
communication,
or any of its contents, is strictly prohibited.  If you have received this
communication in error, please return it to the sender immediately and 
delete the
original message and any copy of it from your computer system.  If you 
have any
questions concerning this message, please contact the sender. 
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Message: 9
Date: Thu, 22 Jul 2010 16:12:56 -0400 (EDT)
From: kgrobert <@t> rci.rutgers.edu
Subject: [Histonet] Sectioning problem
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <8b576f6ad1bfefb4659958e4c7c10cee.squirrel <@t> webmail.rci.rutgers.edu>
Content-Type: text/plain;charset=iso-8859-1

I am working on an old microtome, a Reichert Histostat with a Tissue-Tek
II microtome chuck adapter, and the last time I had this machine cleaned
and rehabbed by my repairman, he said that the springs were shot on the
chuck adapter, resulting in the windowblind chatter that I was getting in
my sections. Unfortunately he did not have a replacement, Sakura doesn't
make or sell them anymore, and the one source for replacement adapters I
did find online sold me a part that had the same problem-shot springs (I
assume this, as I was getting the same chatter with the "new" part).  I
have Googled, searched on eBay, checked online used scientific/medical
equipment suppliers to no avail...but I will keep looking in case
something comes up.

So I guess my question is...would one of the newer cassette holders fit in
my microtomes?  In addition to the Reichert mentioned above, I also have
an American Optical 860.  Or is it possible to figure out how to replace
the springs in the thing? (I have half a mind to wander over to the
mechanical engineering dept. here to ask that question, but I figured I'd
start here on Histonet.) I would really hate to have to replace the
microtomes just because I can't get a cassette holder for them.

Thanks for all your help,

Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914



------------------------------

Message: 10
Date: Thu, 22 Jul 2010 14:02:24 -0700
From: "Bryan Llewellyn" <llewllew <@t> shaw.ca>
Subject: Re: [Histonet] Sectioning problem
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <9CAE5B0417C54C3D9F56AB57B40271D0 <@t> BryanPC>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
    reply-type=original

I had spring problems on the block holder of a microtome many years ago.  I 
solved it by getting a local engineering shop to make me some new springs 
using the old springs as a template.  It worked fine, and was cheap, too.

Bryan Llewellyn


----- Original Message ----- 
From: <kgrobert <@t> rci.rutgers.edu>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, July 22, 2010 1:12 PM
Subject: [Histonet] Sectioning problem


I am working on an old microtome, a Reichert Histostat with a Tissue-Tek
II microtome chuck adapter, and the last time I had this machine cleaned
and rehabbed by my repairman, he said that the springs were shot on the
chuck adapter, resulting in the windowblind chatter that I was getting in
my sections. Unfortunately he did not have a replacement, Sakura doesn't
make or sell them anymore, and the one source for replacement adapters I
did find online sold me a part that had the same problem-shot springs (I
assume this, as I was getting the same chatter with the "new" part).  I
have Googled, searched on eBay, checked online used scientific/medical
equipment suppliers to no avail...but I will keep looking in case
something comes up.

So I guess my question is...would one of the newer cassette holders fit in
my microtomes?  In addition to the Reichert mentioned above, I also have
an American Optical 860.  Or is it possible to figure out how to replace
the springs in the thing? (I have half a mind to wander over to the
mechanical engineering dept. here to ask that question, but I figured I'd
start here on Histonet.) I would really hate to have to replace the
microtomes just because I can't get a cassette holder for them.

Thanks for all your help,

Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology & Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(732) 445-6914

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 11
Date: Thu, 22 Jul 2010 15:40:45 -0700
From: "Morken, Tim" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] Staining dish for vertical 30-slide rack
To: "histonet <@t> lists.utsouthwestern.edu"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <1AAF670737F193429070841C6B2ADD4C023D27BD4D <@t> EXMBMCB15.ucsfmedicalcenter.org>
    
Content-Type: text/plain; charset=us-ascii

Does anyone know of a plastic, inexpensive staining dish for the Richard-Allan or Raymond Lamb 30-slide vertical rack. Something similar to the tissue tek plastic stain dish. The Rack is 11cm / 4 3/8" long


Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
Box 1656
1600 Divisadero St.
San Francisco, CA 94143-1656
USA

Phone: (415) 514-6042
Pager: (415) 443-6509
Fax: (415) 885-7409

Email: tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>



------------------------------

Message: 12
Date: Fri, 23 Jul 2010 08:04:48 -0400
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] biohazard bags
To: "Stacy McLaughlin" <Stacy_McLaughlin <@t> cooley-dickinson.org>, "Jeff
    Birkner" <jbirkner <@t> colabserv.com>,  <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <E78340C766A5284D999F5F5891DDF890144745EE <@t> smcmail.somerset-healthcare.com>
    
Content-Type: text/plain;  charset="utf-8"

I agree. We never re-use them at my facility.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Stacy
McLaughlin
Sent: Thursday, July 22, 2010 3:38 PM
To: Jeff Birkner; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] biohazard bags


Re-use of specimen transport bio-bags is a big NO-NO in my laboratory.  You run the risk of accidentally using one that is contaminated. (This actually happened, and it was not a pretty sight.) 
You can't guarantee that the outside of that specimen container in the bag is not contaminated, even though it looks clean.  You don't know the practices of the person that collected it.  This could be a big liability and the possible consequences just aren't worth it.  
Just my 2 cents.
Stacy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Jeff
Birkner
Sent: Thursday, July 22, 2010 3:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] biohazard bags


We are looking into our current use of biohazard bags.  How many of you
are currently re-using any bags for specimen transport?  These would
only be bags were the actual specimen was inside a secondary container
and thus never directly came into contact with the bags.  Thanks!

 

Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Suite 102
Ottumwa, IA  52501
641-455-5414
ORHC Extension #3538
jbirkner <@t> colabserv.com

 


The information contained in this communication may be confidential, is intended
only for the use of the recipient(s) named above, and may be legally privileged.
If the reader of this message is not the intended recipient, you are hereby 
notified that any dissemination, distribution, or copying of this communication,
or any of its contents, is strictly prohibited.  If you have received this
communication in error, please return it to the sender immediately and delete the
original message and any copy of it from your computer system.  If you have any
questions concerning this message, please contact the sender. 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 13
Date: Fri, 23 Jul 2010 07:15:58 -0700 (PDT)
From: Michelle Mathews <mchelle252002 <@t> yahoo.com>
Subject: [Histonet] p-TBK-1 Ab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <510051.54589.qm <@t> web53308.mail.re2.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Good morning everyone,
I work in a research lab and am trying to optimize a p-TBK-1 antibody; I am 
running this work-up on a BondIII and a BondMax with a Dako Envision Rabbit kit 
for detection and secondary. I have only seen very faint staining and am 
wondering if anyone out there has worked with this and, if so, what type of 
control tissue would show the best signal.
Thanks for any help,
Michelle Mathews, HT(ASCP)
TPL, UNC-CH



      

------------------------------

Message: 14
Date: Fri, 23 Jul 2010 20:56:28 +0530
From: Aazath Raj <aazath <@t> hotmail.com>
Subject: [Histonet] Artifacts in histology section
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT132-w57FCD77C239976388CFD03C8A30 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"



 Dear Friends,

               I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every   day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section.

 

 

with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aazath <@t> hotmail.com

 
                      
_________________________________________________________________
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------------------------------

Message: 15
Date: Fri, 23 Jul 2010 11:34:59 -0400
From: "Figliuolo, Leticia" <leticia.figliuolo <@t> roche.com>
Subject: [Histonet] Microwave Decalcification
To: "'histonet <@t> lists.utsouthwestern.edu'"
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <069E6CF048B915488720412DAFD1474D01BA5D9AC7 <@t> RNUMSEM702.nala.roche.com>
Content-Type: text/plain; charset="us-ascii"

Hello all,

We inherited a Pelco Biowave 34700 (from Ted Pella) and would like to optimize decalcification of rats knee joints, dogs rib using the Biowave. I was wondering if anyone have a protocol for decalcification using nitric acid or formic acid or EDTA using microwave or if you know someone that uses microwave technology for decalcification that I can ask.

Your help is appreciated.
Leticia


------------------------------

Message: 16
Date: Fri, 23 Jul 2010 08:59:06 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Artifacts in histology section
To: histonet <@t> lists.utsouthwestern.edu, Aazath Raj <aazath <@t> hotmail.com>
Message-ID: <902127.95344.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Of the 3 probable causes, the most likely to be is the blade edge not uniformly sharp
René J.
--- On Fri, 7/23/10, Aazath Raj <aazath <@t> hotmail.com> wrote:


From: Aazath Raj <aazath <@t> hotmail.com>
Subject: [Histonet] Artifacts in histology section
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, July 23, 2010, 11:26 AM




Dear Friends,

               I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is that because of blades or due to micro-crystallization of wax or due to any processing problem. Its not consistently in all but i get it on some blocks every   day. Can any one help me in sorting it out. If anybody is interested in will send the picture of those section.





with regards,

Aazathraj.P

Technical Officer,

Apollo Hospitals-chennai

India.

aazath <@t> hotmail.com


                          
_________________________________________________________________
The latest in fashion and style in MSN Lifestyle
http://lifestyle.in.msn.com/_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 17
Date: Fri, 23 Jul 2010 09:09:52 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] artifacts of sectioning
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD034A5488 <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii

It sounds like what you are describing is what we call "chatter."  it
seems to occur most frequently with GI biopsies, esp colon polyps and
usually in the abnormal epithelium.  My techs tell me that cooling the
block better on the ice block makes it go away.  i have no personal
experience, other than to say that when it occurs, it makes the slide of
very poor quality.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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------------------------------

Message: 18
Date: Fri, 23 Jul 2010 17:15:05 +0100
From: "Weaver, Colin" <c.weaver <@t> vla.defra.gsi.gov.uk>
Subject: [Histonet] Formalin substitutes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <0A1C03FC4E63EE4AA24A7983E91C25B502938034 <@t> vla-exchn1.cvlnt.vla.gov.uk>
Content-Type: text/plain; charset="us-ascii"

Hi everyone - does anyone know how effective these formalin substitutes
are at killing microorgansms9esp HG3) in the tissue fixed? I know that
Finefix states that because of the alcohol content being over 70% that
it is effective against many micro-organisms but info seems scarce on
the others

 

Thanks and have a nice weekend

 

Colin Weaver

Histology lab manager

VLA

Thirsk

North Yorkshire

UK

</pre><br>Veterinary Laboratories Agency (VLA)<br><br>This email and any attachments is intended for the named<br>recipient only.<br>If you have received it in error you have no authority to use,<br>disclose, store or copy any of its contents and you should destroy<br>it and inform the sender.
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------------------------------

Message: 19
Date: Fri, 23 Jul 2010 09:20:34 -0700
From: "Matt Brooks" <mbrooks <@t> incytepathology.com>
Subject: RE: [Histonet] artifacts of sectioning
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <706224670091FE47997AEF88EFADE7CA01550E89 <@t> EXCHANGE-SRV.PAI.E-PATHOLOGY.COM>
    
Content-Type: text/plain;   charset="us-ascii"

What I have used in the past is a short warm water soak, 10-15 seconds,
in the water bath, after facing in and between sections, then placing
the block back on ice. I would use a small 200 mL beaker filled with DI
water sitting inside the water, it helps reduce junk floating the bath.
Be careful to avoid over soaking. 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks <@t> incytepathology.com
509-892-2744


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Friday, July 23, 2010 9:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] artifacts of sectioning

It sounds like what you are describing is what we call "chatter."  it
seems to occur most frequently with GI biopsies, esp colon polyps and
usually in the abnormal epithelium.  My techs tell me that cooling the
block better on the ice block makes it go away.  i have no personal
experience, other than to say that when it occurs, it makes the slide of
very poor quality.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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------------------------------

Message: 20
Date: Fri, 23 Jul 2010 10:25:29 -0600
From: Rick.Garnhart <@t> memorialhealthsystem.com
Subject: [Histonet] HPV
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
    <OFEC5D0E64.22985806-ON87257769.005A2E9F-87257769.005A39B1 <@t> memorialhealthsystem.com>
    
Content-Type: text/plain; charset=US-ASCII


We are doing the P16 antibody from MTM Labs.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> memorialhealthsystem.com



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