[Histonet] artifacts of sectioning

[Matt Brooks]

What I have used in the past is a short warm water soak, 10-15 seconds,
in the water bath, after facing in and between sections, then placing
the block back on ice. I would use a small 200 mL beaker filled with DI
water sitting inside the water, it helps reduce junk floating the bath.
Be careful to avoid over soaking. 

Matt Brooks, BS, HT (ASCP)
Histology Supervisor
InCyte Pathology
mbrooks <@t> incytepathology.com
509-892-2744


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tench,
Bill
Sent: Friday, July 23, 2010 9:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] artifacts of sectioning

It sounds like what you are describing is what we call "chatter."  it
seems to occur most frequently with GI biopsies, esp colon polyps and
usually in the abnormal epithelium.  My techs tell me that cooling the
block better on the ice block makes it go away.  i have no personal
experience, other than to say that when it occurs, it makes the slide of
very poor quality.
 
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
 

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