[Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER

[Tony Henwood]

James,

If you are using the distilled water for buffering then the strong
buffer salts (eg phosphate, TRIS etc) will result in a solution that
should not be affected by atmospheric CO2. If you dilute the buffer for
use (as is common) then be careful that you do not dilute out the
buffering capacity of the solution. 

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Thursday, 22 July 2010 5:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER



WE ARE HAVING REAL ISSUES WITH THE PH OF THE DISTILLED WATER WE BUY
COMMERCIALLY.   WE PURCHASE A GENERAL DISTILLED WATER (HINKLEY) AND THE
PH OF THE WATER IS CONSISTENTLY LOW 6.0 OR LESS.   WE TRIED A NURSERY
WATER (WITH A FEW MINERALS ADDED FOR TASTE) AND THE PH WAS 7.0 EXACTLY.
HAS ANYBODY ELSE EXPERIENCED PROBLEMS WITH THE BUFFER PH BECAUSE OF
DISTILLED WATER PH AND DOES ANYBODY HAVE ANY SUGGESTIONS?

James Vickroy BS, HT(ASCP)

Surgical  and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046


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