[Histonet] DISTILLED WATER FOR BUFFER FOR AUTOMATED STAINER

[Pat Laurie]

We ran into this a while ago too.  Our milipore water is consistently at
least 1 pH unit lower than 7. Our chemical safety specialist checked this
out and found out that when the water initially comes out of the tap it is
approx.  100% pure.  Since there are no ingredients in the water at all,
there is nothing to act like a buffer which tap water has.  The water will
start to become acidified by being exposed to the CO2 in the air and form
carbonic acid H2CO3 naturally

 CO2 + H2O [image: is in equilibrium with] H2CO3


which pushed the pH down.  We haven't come up with a good solution other
than pHing the buffer after it is made.

Good luck.

On Wed, Jul 21, 2010 at 12:42 PM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com>wrote:

>
> WE ARE HAVING REAL ISSUES WITH THE PH OF THE DISTILLED WATER WE BUY
> COMMERCIALLY.   WE PURCHASE A GENERAL DISTILLED WATER (HINKLEY) AND THE PH
> OF THE WATER IS CONSISTENTLY LOW 6.0 OR LESS.   WE TRIED A NURSERY WATER
> (WITH A FEW MINERALS ADDED FOR TASTE) AND THE PH WAS 7.0 EXACTLY.  HAS
> ANYBODY ELSE EXPERIENCED PROBLEMS WITH THE BUFFER PH BECAUSE OF DISTILLED
> WATER PH AND DOES ANYBODY HAVE ANY SUGGESTIONS?
>
> James Vickroy BS, HT(ASCP)
>
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com