[Histonet] Cryostat Decontamination

malbenatti <@t> gmail.com malbenatti <@t> gmail.com
Wed Jul 21 13:47:02 CDT 2010

Hy Karyrn.

As my lab carry frozen section daily, the decontamination/fumigation of the
cryostat on a daily basis is not practical as we do not have a back up
cryostat. Also after every frozen section, we make sure that the Cryostat is
get disinfected of any fresh tissue trimming with 70 % alcohol.
Every friday night the cryostat get set-up for fumigation and get
decontaminated weekly using Formaldehyde vapour/ then we neutralise them
with ammonia, according to manufacturer recommendation (Thermo Scientific),
though the fumigation will not activate until Sunday night so Week-end
On-Call staff can carry urgent Frozen sections if needed.  Each
decontamination/fumigation cycle is then Log, for CPA purposes.

Hope this helps


---------- Forwarded message ----------
From: "Myers, Karyn" <KarynMyers <@t> texashealth.org>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu
Date: Wed, 21 Jul 2010 08:57:35 -0500
Subject: [Histonet] Cryostat Decontamination

Our Hospital has several Frozen Section Rooms, each of which have 1 to 2
The majority of our cryostats are the newer models, with the built in
Ultraviolet Radiation Disinfection cycle (runs every afternoon.)  However,
we still have a couple of the older models that do not have an automatic
disinfection cycle.    What are other institutions currently doing to
disinfect their cryostats.
Any information/policy will be greatly appreciated,

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