[Histonet] decontamination of cryostat

annigyg <@t> gmail.com annigyg <@t> gmail.com
Wed Jul 21 11:23:35 CDT 2010

If I may ask, what do you contaminate with when doing a full meltdown defrost clean up after a suspect TB or hep B pos case?
Some proprietary decontaminant?
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-----Original Message-----
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Wed, 21 Jul 2010 09:01:19 
To: Tench, Bill<Bill.Tench <@t> pph.org>; <histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] decontamination of cryostat

Bill and company

This is a very difficult, there are times were decontamination is needed
immediately depending on the outcome of the frozen or there is scheduled
maintenance that needs to occur with the decontamination as well.  The
UV light is not sufficient enough to call this decontamination.  CAP has
stated that it needs a specific reagent used in this decontamination
procedure.  My Chair was upset by this statement from the College and
called them to ask for clarification.  The main purpose is documented
and scheduled times of decontamination is what we received back from the
College.  Of course there are circumstances when a case presents itself,
action needs to be taken and documented.  I would say is look at
creating a scheduled decontamination that is appropriate for you
facility.  We have elected for a monthly Decontamination of cryostats,
this is based on usage and history.  This is documented and the
appropriate solution is used during decontamination.  This is done
during the normally work day since we have two cryostats.  This usually
takes 3 days to do right, but some facilities might not be able to do

Jesus Ellin
Yuma Regional Medical Center

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tench,
Sent: Wednesday, July 21, 2010 8:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] decontamination of cryostat

I would suggest that if you are a CAP certified lab that you read the
appropriate section in the current LAP standards list.  The note
associated with the standard is pretty specifiic.  I believe that if you
use the cryostat daily, you need to decontaminate once a week, but i
don't have the standard available.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California  92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604

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