[Histonet] human on human IHC

[sgoebel <@t> xbiotech.com]

   Anything  using  FITC  is  pretty  expensive.  If you aren't want   flourescence  then  why  would you use this?  Does this specific conj   ugation  eliminate the Fc binding?  Conjugating to HRP doesn't seem to
      que
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician
   <
   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 
   Austin, Texas  78744

   
   (512)386-5107
   -------- Original Message --------
   Subject: Re: [Histonet] human on human IHC
   From: [1]andreahooper <@t> rocketm   Date: Mon, July 19, 2010 6:06 am
   To:  [2]sgoebel <@t> xbiotech.com,  "Ki   oo.com>
   Cc: "Histonet" <[4]h   Along  those  same  lines I have conjugated primaries to biotin and it
   works  b   extra  amplificati   using a rabbit-anti-FITC fo   Just  be  aware with human on human controls are imperative as you may
   get bi   Andrea Hooper
   Sent from my Verizon Wireless BlackBerry
   -----Original Message-----
   From: <[5]sgoebel <@t> xbiotech.com>
   Sender: [6]hist   Date: Thu, 15 Jul 2010 09:27:19
   To: Kim Merriam<[7]kmerriam2003   Cc: Histonet<[8]hist   Subject: RE: [Histonet] human on human IHC
   I am doing alot with this right now. The best thing I havefound to
   do is to conjugate your primary antibody with HRP, then just use
   the direct method (no secondary), and it turns out beautifully!!
   You can buy thekit to do this from Fisher. I can get the exact
   order number if you need it. Good Luck!!
   Sarah Goebel, B.A., HT (ASCP)
   Histotechnician
   XBiotech USA Inc.
   8201 East Riverside Dr. Bldg 4 Suite 100
   Austin, Texas 78744
   (512)386-5107
   -------- Original Message --------
   Subject: [Histonet] human on human IHC
   From: Kim Merriam <[1][9]kme   Date: Thu, July 15, 2010 8:00 am
   To: Histonet <[2][10]histonet <@t> lists.utsouthwestern.edu>
   Hi,
   Does   anyone  know  of  a  human-on-human  IHC  kit  similar  to  the
   mouse-on-mouse kits
   that are available?
   Thanks,
   Kim
   Kim Merriam, MA, HT(ASCP)QIHC
   Cambridge, MA
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References

   1. 3D"mailto://andreahooper@rocketmail.com"/
   2. 3D"mailto://sgoebel@xbiotech.com"/
   3. 3D"mailto://kmerriam2003@yahoo.com"/
   4. 3D"mailto://histonet@lists.utsouthwestern.edu"/
   5. 3D"mailto://sgoebel@xbiotech.com"/
   6. 3D"mailto://histonet-bounces@lists.utsouthwestern.edu"/
   7. 3D"mailto://kmerriam2003@yahoo.com"/
   8. 3D"mailto://histonet@lists.utsouthwestern.edu"/
   9. 3D"mailto://kmerriam2003@yahoo.com"/
  10. 3D"mailto://histonet@lists.utsouthwestern.ed=/
  11. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
  12. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"  13. 3D"mailto://kmerriam2003@yahoo.com"/
  14. 3D"mailto://histonet@lists.utsouthwestern.edu"/
  15. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
  16. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
  17. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"