[Histonet] Lyve - 1 on Bond Max

[Malika Benatti]

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Hi there,

I was wondering if anyone on the histonet list use anti-mLYVE-1 antibody (purified in rat Monoclonal IgG 2A from R&D System) on the Bond Max.
We have been trying out various titrations and pre-treatment without much success.
All we seems to achieve is background staining with no real specificity at 
1:100 H1(10) ; 1:100 H2(20) ;1:100 E2(10): 1:50 E1(30) ;1:50 H1(30); 1:50 H2(10)

Any suggestion ?

Regards

Malika










Malika Benatti
 
Specialist BMS 
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH

 
Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875
 
benatm <@t> gosh.nhs.uk


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