[Histonet] Re: NG2 antibody/ Antigen retrieval/Protocol
carl.hobbs <@t> kcl.ac.uk
Fri Jul 16 14:06:11 CDT 2010
"I am trying to stain human glioma sections(as a positive control) using Anti-NG2 antibody. The tissue that I have is Paraffin embedded and I appreciate if somebody could send me the protocol for antigen retrieval ,antibody titer and IHC protocol. "
Let us know what Ab you are currently using ( Source and catalogue number) and also what detection/AR protocol you are using.
Also, what problem do you have using your Ab?
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