[Histonet] Hemoglobin Stain

[Anna Taylor]

Hi Linda,


Thanks for the reply! No, I'm not limited to staining only. In fact, I will be performing some seperate IHC to demonstrate a couple of other proteins. It is simply my preference to use a histochemical technique for the following reasons. Firstly, from what I understand, staining will not be as laborious as IHC nor as difficult/technical to obtain positive results. Secondly, as this is part of my Honours year project, I would like to try my luck with several different techniques, and hemoglobin is the only molecule from the products I am looking at for which a simple histochemical approach is possible. Again, any suggestions would be highly valuable.


Cheers,
Anna

> Subject: RE: [Histonet] Hemoglobin Stain
> Date: Thu, 15 Jul 2010 11:41:54 -0500
> From: LSebree <@t> uwhealth.org
> To: annataylor <@t> hotmail.com; histonet <@t> lists.utsouthwestern.edu
> 
> Anna,
> 
> Are you limited to histochemical stains?  Because if you're not,
> hemoglobin may be detected by immunohistochemistry quite nicely. 
> 
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> DB1-223 VAH
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anna
> Taylor
> Sent: Thursday, July 15, 2010 11:37 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Hemoglobin Stain
> 
> 
> Hi all,
> 
> In my quest for advice about what will be my very first attempt at
> histology, I have been recommended to post my query here.
> 
> I'm attempting to demonstrate hemoglobin in FFPE lymph node sections.
> I've been able to find a couple of techniques  (namely the Dunn &
> Thompson technique and Okajima's technique), although I'm not sure how
> suitable either would be on lymph node sections? As I said, this will be
> my debut experience in histology/histochemistry so any
> advice/recommendations at all will be appreciated :)Thanks,Anna.
>  		 	   		  
> _________________________________________________________________
> View photos of singles in your area! Looking for a hot date?
> http://clk.atdmt.com/NMN/go/150855801/direct/01/________________________
> _______________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  
_________________________________________________________________
If It Exists, You'll Find it on SEEK. Australia's #1 job site
http://clk.atdmt.com/NMN/go/157639755/direct/01/