[Histonet] Resin embedded tissue
ratliffjack <@t> hotmail.com
Thu Jul 15 07:17:59 CDT 2010
I use just regular glass slides, coat them with Haupt's adhesive, roll the sections flat and then press and dry the sections to the coated slide using a slide press and oven at 50C. In 13 years of hard tissue resin histology, I have never (knocking twice on wood) lost a section or had a section lift up in any area during staining using this method. You will get a degree of background with certain stains (hematoxylin, analine blue, etc) because it is a gelatin based adhesive, but these problems are easily resolved with a little overstaining and acid alcohol rinse.
If interested you can get the Haupt's and slide press from Dorn and Hart Microedge (www.dornandhart.com). I have also been told that they have a couple of new kits coming out by the end of the summer - a resin embedding kit (using Perkadox as a catalyst) and thin section microtomy kit (one for a rotary microtome and one for a sledge or Polycut microtome) that includes everything needed to section resin blocks. Look them up and contact Bill Hart for more information.
On Jul 15, 2010, at 3:09 AM, "Tilston, Valerie" <V.Tilston <@t> liverpool.ac.uk> wrote:
> Does anyone have experience of cutting resin embedded bone and if so what type of slides do people use? We are having problems with sections falling off or the bone not remaining flat on the slide!
> Many thanks in advance,
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