[Histonet] Difference between Protease and Proteinase-K?

Kathleen Jones Kjones <@t> upei.ca
Mon Jul 12 12:35:40 CDT 2010

Hello Jennifer
I'm not sure what platform you are using, but from my experience, I
obtain beautiful results for CD31 in canine and feline tissue, but the
protocols for each are quite different. For CD31 in canine tissue I use
a tris based buffer and for feline I use protease. 
I know this doesn't answer you question, but thought I would pass along
what works for me.
Good Luck
Kathleen Jones
Research Technician

>>> "Jennifer Campbell" <jcampbell <@t> vdxpathology.com> 7/12/2010 2:07 PM
Hi All,

  I would like to try enzyme digestion on my CD31 (for K9 and feline
tissue) which I haven't been able to get to work using citrate buffer
6.0 or pH 9.0.  I have heard recommendations for both Protease and
Proteinase-K but, do not know which one I should try out.  Any
on the differences between the two?


Jennifer Campbell
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

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