[Histonet] Difference between Protease and Proteinase-K?

Jennifer Campbell jcampbell <@t> vdxpathology.com
Mon Jul 12 12:07:30 CDT 2010


Hi All,
 
  I would like to try enzyme digestion on my CD31 (for K9 and feline
tissue) which I haven't been able to get to work using citrate buffer pH
6.0 or pH 9.0.  I have heard recommendations for both Protease and
Proteinase-K but, do not know which one I should try out.  Any feedback
on the differences between the two?
 
Thanks,
 
Jennifer Campbell


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