[Histonet] Reprocess

[Tony Henwood]

You do not need to rehydrate the blocks back to water, 

While the tissue is still hot (ie the wax is still molten) blot the
tissue dry, place it back in its cassette and place the cassette in 10%
formalin for reprocessing. This procedure dramatically improves the
results in at least 90% of cases. The excellent results are probably due
to the protective nature of the wax present in the adequately processed
portions of the block. This insulates the tissue from the harmful
effects of ethanol on the adequately processed portions of the tissue
preventing the tissue from becoming hard and brittle (Johnson 2003).

Johnson (2003) Histologic 36(1):21-22.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
sgoebel <@t> xbiotech.com
Sent: Thursday, 8 July 2010 1:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Reprocess



   Hello  all!!  Hope everyone had a happy 4th!!  Question =oday is...I
   grossed  in  some  fat  that I need to routine process.  I ha=e done
   this  in  the  past  with  extra fixation and no problem?  This tim=
   however  it didn't fix all the way through and I have oily unfixed
fat
   in  =the  middle  of  my  block.  I fixed for 48 hours, but guess my
   section  we=re  too big and needed more.  I want to fix for a little
   longer  and rep=ocess the block.  I know I need to melt it down, but
   then  what? =I  have done this before, it's just my brain is getting
   older =)

   Thanks in advance!!

   Sarah Goebel, B.A., HT (=SCP)

   Histotechnician
   
   XBiotech USA Inc.

   
   8201 East Riverside Dr. Bldg 4 Suite 100

   Austin, Texas  78744

   (512)386-5107 _______________________________________________
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