[Histonet] RE: Histonet- Xylene Substitutes

Tue Jul 6 10:17:17 CDT 2010

Vanessa, 
I work with a lot of great histology folks and what I know about it is that
eosin bleeding and blue-haze problems usually occur as a consequence of
either not fully removing all of the water during the final absolute alcohol
rinse step, and/or not fully removing all of the alcohol during the clearing
step.  If the water and alcohol are completely removed, bleeding and haze
will not occur. I hope this helps. 

Beth Austin-Sell
CBG Biotech

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Subject: Histonet Digest, Vol 80, Issue 4

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Today's Topics:

   1. Re: control tissue for Hall's bile stain (Robert Richmond)
   2. RE: BILLING QUESTION (Cynthia Pyse)
   3. RE: ER and PR validation (JMyers1 <@t> aol.com)
   4. Thomas Crowell is out of the office. (thomas.crowell <@t> novartis.com)
   5. Looking for MIN6 cell line (Debra Baluch)
   6. bat wing histology (Amos Brooks)
   7. RE: BILLING QUESTION (Feher, Stephen)
   8. xylene substitute (Vanessa Avalos)

----------------------------------------------------------------------

Message: 1
Date: Fri, 2 Jul 2010 13:48:52 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: control tissue for Hall's bile stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTilDU0QIPr9rjajgQqOnoAANF2Yk1skC0hOUKT0C <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hall's bile stain - not really a stain but a reaction, like the Perls
prussian blue reaction for iron - uses trichloracetic acid and ferric
chloride to oxidize yellow-brown bilirubin to bright green biliverdin.
Or so I've been told - I've never seen one.

Something I've wondered about for a long time: could Hall's reaction
be used to demonstrate meconium in macrophages in the chorion of the
human placenta? These can be hard to see, particularly if you don't
have a very good microscope. - If this would work, it would provide a
ready source for controls, at least for the hospital pathologist.

Bob Richmond
Samurai Pathologist
Knoxville TN

------------------------------

Message: 2
Date: Fri, 2 Jul 2010 13:51:51 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] BILLING QUESTION
To: "'Sara Baldwin/mhhcc.org'" <sbaldwin <@t> mhhcc.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cb1a0f$40ca1c10$c25e5430$@com>
Content-Type: text/plain;	charset="us-ascii"

We have a contract with the hospitals, this comes from our billing manger.
We bill the hospitals, then they bill the patient. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Friday, July 02, 2010 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] BILLING QUESTION

Histonetters:
If you do some work for another hospital (Histology) can you bill the
hospital or do you have to bill the patient directly?  Is there a statute or
Regulation out there about this?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin <@t> mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232, 
Pager 812-481-0897
Confidential information, Authorized use only.

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------------------------------

Message: 3
Date: Fri, 2 Jul 2010 13:54:26 EDT
From: JMyers1 <@t> aol.com
Subject: RE: [Histonet] ER and PR validation
To: histonet <@t> lists.utsouthwestern.edu
Cc: Dorothy.L.Webb <@t> HealthPartners.Com
Message-ID: <8f84.3cbd32f0.395f81d2 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"

This paper can also be downloaded, indirectly, from the CAP's website:

_http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_ 
(http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) 

Happy (re-)validating,
Joe

------------------------------

Message: 12
Date: Fri, 2 Jul 2010 10:23:08 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] ER and PR validation
To: _'histonet <@t> lists.utsouthwestern.edu'_ 
(mailto:'histonet <@t> lists.utsouthwestern.edu') 

In a recent article written by 4 pathologists, including Dr. Elizabeth 
Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including 
testing with a lab that has validated it's assay against clinical outcomes.
How 
is everyone planning on fulfilling this requirement inasmuch as we are not 
aware of any lab in our metro area with this kind of compliance.

Thanks, as always, for any guidance in this area!  If you would like a 
reprint of this article, email is pfitz <@t> stjoe.org and the article is entitle

"Recommendations for Validating Estrogen and Progesterone Receptor 
immunohistochemistry Assays".

Dorothy Webb, HT
Regions Histology Technical Supervisor

------------------------------

Message: 4
Date: Fri, 2 Jul 2010 15:32:28 -0400
From: thomas.crowell <@t> novartis.com
Subject: [Histonet] Thomas Crowell is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:

<OF9A757D25.2860015A-ON85257754.006B57CD-85257754.006B57CD <@t> ah.novartis.com>

Content-Type: text/plain; charset=US-ASCII

I will be out of the office starting  07/02/2010 and will not return until
07/13/2010.

Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.

------------------------------

Message: 5
Date: Fri, 2 Jul 2010 13:14:00 -0700
From: Debra Baluch <PAGE.BALUCH <@t> asu.edu>
Subject: [Histonet] Looking for MIN6 cell line
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<80BBC916BEC29A498784913D007DD4A24DBDC0A1FD <@t> EX10.asurite.ad.asu.edu>
Content-Type: text/plain; charset="us-ascii"

Does anyone know where I can obtain the MIN6 beta cell line derived from
mouse pancreas? They are listed and used in many papers but do not list the
source. I have tried ATCC and suggested cell banks in ECACC (England), DSMZ
(Germany), and JCRB (Japan) but they are not listed. 

Thanks,

Page
Arizona State University/SoLS

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
JMyers1 <@t> aol.com
Sent: Friday, July 02, 2010 10:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Dorothy.L.Webb <@t> HealthPartners.Com
Subject: RE: [Histonet] ER and PR validation

This paper can also be downloaded, indirectly, from the CAP's website:

_http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48_
(http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165-134.7.e48) 

Happy (re-)validating,
Joe

------------------------------

Message: 12
Date: Fri, 2 Jul 2010 10:23:08 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] ER and PR validation
To: _'histonet <@t> lists.utsouthwestern.edu'_
(mailto:'histonet <@t> lists.utsouthwestern.edu') 

In a recent article written by 4 pathologists, including Dr. Elizabeth
Hammond and Dr. Patrick Fitzgibbons, the recommendations are now including
testing with a lab that has validated it's assay against clinical outcomes.
How is everyone planning on fulfilling this requirement inasmuch as we are
not aware of any lab in our metro area with this kind of compliance.

Thanks, as always, for any guidance in this area!  If you would like a
reprint of this article, email is pfitz <@t> stjoe.org and the article is entitle
"Recommendations for Validating Estrogen and Progesterone Receptor
immunohistochemistry Assays".

Dorothy Webb, HT
Regions Histology Technical Supervisor
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 6
Date: Fri, 2 Jul 2010 16:14:36 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] bat wing histology
To: mchadha1 <@t> gmail.com, histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTikzxrY16AvVVFHaOHPsgHMYS-iDA0MIVTeMCpKJ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi,
    I do hope you are looking at cross sections of the wing and not the
flat. That would be very difficult indeed. For good cross sections I would
try a "Swiss Roll". This is a way of demonstrating a large amount of cross
sectional area in small space. Take the membrane and fix it by submersion in
the fixative of your choice. Prior to processing roll the whole membrane up
then cut the membrane log into sections small enough to fit in a cassette.
You can use foam biopsy pads to support this shape. Embed it and section it
on edge to show a long coiled membrane. The hairs should be able to be
displayed in this way as well. To show a lot of membrane at the same time
you could place multiple rolls in one cassette. This should work well.

Good Luck,
Amos

Message: 21
Date: Thu, 1 Jul 2010 11:46:17 -0400
From: Mohit Chadha <mchadha1 <@t> gmail.com>
Subject: [Histonet] bat wing histology
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
       <AANLkTikKGXt1_uY5qkdVhP04fTyIUOCWY9XNVBlEQgW4 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello everyone,

This is my very first post and I am desperately looking for help. I am new
to histology, so any help would be much appreciated.

I am studying the peripheral sensory innervation of bat wings. As a first
step, I would like to demonstrate the innervation pattern on the different
parts of the wing membrane (a whole mount of the wing?). Second, I would
like to demonstrate the mechanoreceptor make-up of the tiny hairs on the
wing membrane.

Bat wings are highly elastic, with numerous folds, a thickness of about
35-45 microns (in the species I study), a network of thin collagen bundles,
and pigmented superficial epidermal layers.
I could provide more information if required.

Hoping to hear back from the members.
Thank you.

------------------------------

Message: 7
Date: Fri, 2 Jul 2010 17:18:14 -0400
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] BILLING QUESTION
To: "Cynthia Pyse" <cpyse <@t> x-celllab.com>,	"Sara Baldwin/mhhcc.org"
	<sbaldwin <@t> mhhcc.org>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<73A7ED895EE0C24D9267ED814911DF1912B75578 <@t> exchange.cmc-nh.org>
Content-Type: text/plain;	charset="us-ascii"

You can contract for services to another lab or hospital and depending
upon the terms of the contract, you may be able to bill the patient.
For example, you send all of your HER2's and ER/PR to another lab to be
stained and quantified into a score.  You may contract with the other
lab for them to charge you a flat fee for the service and you will bill
the patient for 88361x3.  

The other side is that you can contract for the other hospital to do the
test, bill the patient and your hospital pays no fee but misses out on
some of the compensation. 

Steve

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia
Pyse
Sent: Friday, July 02, 2010 1:52 PM
To: 'Sara Baldwin/mhhcc.org'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] BILLING QUESTION

We have a contract with the hospitals, this comes from our billing
manger.
We bill the hospitals, then they bill the patient. 

Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sara
Baldwin/mhhcc.org
Sent: Friday, July 02, 2010 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] BILLING QUESTION

Histonetters:
If you do some work for another hospital (Histology) can you bill the
hospital or do you have to bill the patient directly?  Is there a
statute or Regulation out there about this?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center sbaldwin <@t> mhhcc.org Ph
812-482-0210, 482-0216,  Fax 812-482-0232, Pager 812-481-0897
Confidential information, Authorized use only.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 8
Date: Fri, 2 Jul 2010 15:20:52 -0700
From: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>
Subject: [Histonet] xylene substitute
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000301cb1a34$dbdd5c10$93981430$@com>
Content-Type: text/plain;	charset="us-ascii"

I am taking a chance that this is still a working email!! I came across an
old post on histonet regarding Xylene subs with a linear stainer. I was
wondering if you made the switch, and if so could you share the brand and
your H&E protocol. I have had some issues with Eosin  bleeding, slides very
eosinophilic and blue haze on the slide. Not all at once but its enough to
drive me crazy!! Your help would be appreciated. 

V.Avalos

ADS, INC

Fax:602-277-2134

------------------------------

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