SPAM-LOW: Re: [Histonet] Unstained Slides

Patsy Ruegg pruegg <@t>
Sun Jul 4 11:57:39 CDT 2010

I do not bake my unstained slides for IHC controls and store them at 4dc.  I
bake them just before use.  They do last longer this way but Richard is
right, there is no telling because it all depends on the fixation, target
protein stability and storage conditions.  It is always a good idea to cut
fresh sections if the stored unstained sections do not perform as expected.


Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Richard
Sent: Monday, June 28, 2010 10:55 AM
To: histonet <@t>;
Rick.Garnhart <@t>
Subject: SPAM-LOW: Re: [Histonet] Unstained Slides

We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT.
There is no set rule for stability.  It all depends on fixation, the nature
of the protein target, and storage conditions.  I've seen absolutely
spectacular immunoreactivity on unstained slides stored at RT for 20 years
(and longer) and I've seen reduced immunoreactivity in unstained slides
stored for as little as 4 weeks.  We will always attempt to stain unstained
slides when available; however, if the lesion or tumor is negative, and
there is no internal  control, you better cut fresh sections.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax

>>> <Rick.Garnhart <@t>> 6/28/2010 11:54 AM >>>

Histoland, How  is everyone storing/filing unstained slide. And how long are
they good for to use for immunohistochemistry.

Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> 

Mission: To provide the highest quality health care
Vision: To create an outstanding health system where patients heal and
people thrive
Values: Compassion - Integrity - Quality - Respect - Teamwork 

The information contained in or attached to this electronic message is
privileged and confidential, intended only for the use of the individual(s)
named above. If the reader of this message is not the intended recipient, or
the employee or agent responsible to deliver it to the intended
recipient, you are hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you have received
this communication in error, please inform the sender immediately and remove
any record of this message._______________________________________________
Histonet mailing list
Histonet <@t>

Histonet mailing list
Histonet <@t>

More information about the Histonet mailing list