SPAM-LOW: Re: [Histonet] Unstained Slides

[Patsy Ruegg]

I do not bake my unstained slides for IHC controls and store them at 4dc.  I
bake them just before use.  They do last longer this way but Richard is
right, there is no telling because it all depends on the fixation, target
protein stability and storage conditions.  It is always a good idea to cut
fresh sections if the stored unstained sections do not perform as expected.

Regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent: Monday, June 28, 2010 10:55 AM
To: histonet <@t> lists.utsouthwestern.edu;
Rick.Garnhart <@t> memorialhealthsystem.com
Subject: SPAM-LOW: Re: [Histonet] Unstained Slides

We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT.
There is no set rule for stability.  It all depends on fixation, the nature
of the protein target, and storage conditions.  I've seen absolutely
spectacular immunoreactivity on unstained slides stored at RT for 20 years
(and longer) and I've seen reduced immunoreactivity in unstained slides
stored for as little as 4 weeks.  We will always attempt to stain unstained
slides when available; however, if the lesion or tumor is negative, and
there is no internal  control, you better cut fresh sections.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> <Rick.Garnhart <@t> memorialhealthsystem.com> 6/28/2010 11:54 AM >>>

Histoland, How  is everyone storing/filing unstained slide. And how long are
they good for to use for immunohistochemistry.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> memorialhealthsystem.com 



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