[Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31

[Patsy Ruegg]

Thank you Kim, that does sound very promising, I still have not gotten
around to trying my sample of this, your work will be very helpful when I
do.

 

Best regards,

 

Patsy 

 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

 


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From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
James Watson
Sent: Thursday, January 28, 2010 8:19 AM
To: 'kmerriam2003 <@t> yahoo.com'; Histonet; ihcrg <@t> googlegroups.com
Subject: RE: [IHCRG] Dianova rat anti-mouse CD31

 

I just got some of this antibody in an will be setting it up on the Ventana
Discovery Thank you for the information on your protocol.  I will let you
know how it goes.

 

Jamie

 

James Watson HT  ASCP

Facilities Manager of Histology

GNF  Genomics Institute of the Novartis Research Foundation

Tel    858-332-4647

Fax   858-812-1915 <mailto:858-812-1915jwatson <@t> gnf.org> 

jwatson <@t> gnf.org

 

 

From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
Kim Merriam
Sent: Thursday, January 28, 2010 6:46 AM
To: Histonet; ihcrg <@t> googlegroups.com
Subject: Fw: [IHCRG] Dianova rat anti-mouse CD31

 

Hi - someone emailed me about my methodology, but I cant find the email, so
here it is:
 

I tried it @ 1:10, 1:25 and 1:50.  All seem viable dilutions for the FFPE
xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary
ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer kit (15 minutes each)
+ DAB.  The path labs (for my company) that are in California and Seattle
used the same retreival but different detection methods and got similar
results.

 

Kim

 

From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
Kim Merriam
Sent: Wednesday, January 27, 2010 9:23 AM
To: Histonet; ihcrg <@t> googlegroups.com
Subject: [IHCRG] Dianova rat anti-mouse CD31

 

Hi All,

 

I know there was some buzz a while back about this antibody.  I have tested
it on mouse FFPE xenografts, and I must say, it looks very promising.  I
compared 2 pieces of the same mouse xenograft; one half of the tumor was
fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31
and the other half of the tumor was fixed in NBF.  The NBF-fixed tumor
actually looks better!

 

A couple of other folks that are working at the west coast sites of my
company have had similar results.  We are all very encouraged.

 

I still need to do a bit more testing with some other tissues (and perhaps
some additional titrations), but I thought I would pass this along to
everyone.

 

Kim
 

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA