[Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31
James Watson
JWatson <@t> gnf.org
Thu Jan 28 09:18:32 CST 2010
I just got some of this antibody in an will be setting it up on the Ventana Discovery Thank you for the information on your protocol. I will let you know how it goes.
Jamie
James Watson HT ASCP
Facilities Manager of Histology
GNF Genomics Institute of the Novartis Research Foundation
Tel 858-332-4647
Fax 858-812-1915<mailto:858-812-1915jwatson <@t> gnf.org>
jwatson <@t> gnf.org<mailto:jwatson <@t> gnf.org>
From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of Kim Merriam
Sent: Thursday, January 28, 2010 6:46 AM
To: Histonet; ihcrg <@t> googlegroups.com
Subject: Fw: [IHCRG] Dianova rat anti-mouse CD31
Hi - someone emailed me about my methodology, but I cant find the email, so here it is:
I tried it @ 1:10, 1:25 and 1:50. All seem viable dilutions for the FFPE xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary ab for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer kit (15 minutes each) + DAB. The path labs (for my company) that are in California and Seattle used the same retreival but different detection methods and got similar results.
Kim
From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of Kim Merriam
Sent: Wednesday, January 27, 2010 9:23 AM
To: Histonet; ihcrg <@t> googlegroups.com
Subject: [IHCRG] Dianova rat anti-mouse CD31
Hi All,
I know there was some buzz a while back about this antibody. I have tested it on mouse FFPE xenografts, and I must say, it looks very promising. I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF. The NBF-fixed tumor actually looks better!
A couple of other folks that are working at the west coast sites of my company have had similar results. We are all very encouraged.
I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone.
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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