[Histonet] RE: Histonet Digest, Vol 74, Issue 32

Wed Jan 27 11:49:42 CST 2010

Hi all
Have you looked into the BioImagene Concerto scanner? This scanner can provide dark feld and brightfield image analysis in addition to the telepathology, frozen section collaboration capability, education archival, and the works. This scanner, from what I hear is already available or will be available in March.
The rate of scan, the image analysis and software package (virtuoso software package) is quite amazing! And there is NOTHING else in the marketplace
Check out the website: bioimagene.com
For more details 

T Oeler

No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced.
Theresa A Oeler, BS
Senior Research Histologist
NSABP Pathology Laboratory
Federal North Building
1307 Federal Street, Suite 303
Pittsburgh, PA 15212
412/359-8931 Of.  412/359-3239 Fx.
theresa.oeler <@t> nsabp.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, January 27, 2010 11:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 74, Issue 32

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Today's Topics:

   1. Coverslipping (Breeden, Sara)
   2. Peloris and Bond service (Rathborne, Toni)
   3. 20% NaOH Nail pre-Treatment... Questions (Due, Brice)
   4. Re: Diff-Quik (Robert Richmond)
   5. RE: Re: Diff-Quik (Tony Henwood)
   6. Re: Histology Special Stains for Macrophages (John Kiernan)
   7. Re: removing trigeminal nerve and ganglion (John Kiernan)
   8. Re: Coverslipping (John Kiernan)
   9. Coverslipping (mtitford <@t> aol.com)
  10. Coverslipping Photo Thanks (Breeden, Sara)
  11. Dako vs Ventana (kristen arvidson)
  12. RE: Dako vs Ventana (Blazek, Linda)
  13. Hamamatsu Nanozoomer (Kim Merriam)
  14. Re: Dako vs Ventana (Mark Tarango)
  15. Dianova rat anti-mouse CD31 (Kim Merriam)
  16. RE: [IHCRG] Dianova rat anti-mouse CD31 (Baldridge, Lee Ann)
  17. Re: Re: Diff-Quik (Kim.Donadio <@t> bhcpns.org)
  18. RE: Re: Diff-Quik (Mike Pence)
  19. RE: Dako vs Ventana (Mahoney,Janice A)
  20. RE: Re: Diff-Quik (Kim.Donadio <@t> bhcpns.org)
  21. Pathology Laboratory Manager Opening in Houston, Texas
      (Cory Collins)

----------------------------------------------------------------------

Message: 1
Date: Tue, 26 Jan 2010 12:09:51 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Coverslipping
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4D14F0FC9316DD41972D5F03C070908B02E46DC7 <@t> nmdamailsvr.nmda.ad.nmsu.edu>

Content-Type: text/plain;	charset="us-ascii"

Do any of my Esteemed Colleagues have a photo somewhere of someone
hand-coverslipping?  I need a picture of a "normal" hand coverslipping
(putting coverslip ON slide instead of slide ON coverslip).  Just one
photo is all I need.  I'm trying to describe in words how to do this and
it's not easy!  Thanks in advance for your Digital Donation (and I mean
"electronic" digital, so don't give me any flak).  If you can email it,
I can pass it along.

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

------------------------------

Message: 2
Date: Tue, 26 Jan 2010 14:15:42 -0500
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: [Histonet] Peloris and Bond service
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E78340C766A5284D999F5F5891DDF8900BAF918D <@t> smcmail.somerset-healthcare.com>

Content-Type: text/plain;  charset="utf-8"

We're looking into service agreements for these instruments. Can anyone who currently uses them help me decide which Leica service contract would be best? How often do you experience problems with these instruments that you need to have service for them? What is your volume? Could those service calls have been avoided if you had a/another pm done?

Thank you in advance!
Toni 

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------------------------------

Message: 3
Date: Tue, 26 Jan 2010 18:05:40 -0500
From: "Due, Brice" <BDUE <@t> PARTNERS.ORG>
Subject: [Histonet] 20% NaOH Nail pre-Treatment... Questions
To: "Joe \"The Toe\" Nocito" <jnocito <@t> satx.rr.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<F88EDAB7E59CFE4FA90586EBAF2CBCFF0CA891 <@t> PHSXMB38.partners.org>
Content-Type: text/plain; charset="us-ascii"

Hello Joe, first thanks so much for sharing your expertise here on Histonet.

I work in the main histo lab at Mass General Hosp. in Boston and I have
interested the powers that be in your NaOH protocol. I initially used your NaOH
idea to attach an "impossible" nail to slides by floating 4um section on 20%
NaOH for 10-15min. (The transfers between regular water bath and NaOH bath (and
back to rinse) were surprisingly easy because the concentrated NaOH prevents
adhesion to even the stickiest adhesive slides.)

Anyhow, I have some questions for you about your lab's implementation. I found
your most complete post at
http://lists.utsouthwestern.edu/pipermail/histonet/2008-October/040078.html

But I am concerned about the safety of 20% NaOH in routine use. Would you mind
posting or emailing me any SOPs you have that address the safety issues? 

Specifically both the histology lab and the "grossing" labs here use RDO decal
solutions at every bench. So there is/will be concern about the proper handling
of 20% NaOH around RDO... by overworked PAs and residents, etc. etc. Feel free
to tell me your safety almost-horror stories so we can (try to) prevent them
from occuring here.

I've seen that some people use 10% NaOH instead. Have you experimented with
this? Any reasons you stick with 20% beyond the usual "it ain't broke, so..."?
Have you (or anyone on Histonet) ever tried to find the minimum effective
concentration? Increasing the pre-soak time isn't an issue here since I've been
assured that nail turnaround times are non-critical.

Along these lines, do you or anyone have any histochemical references for the
reactions that are taking place? With this info it might be possible to predict
the minimum effective pH. It would also be nice to have references for the SOP.
(I know about the coverslip "crushes" some derm clinicians do with fresh
scrapings. Alternately, do you know of any histochemical references for this?)

Thank you so much for sharing your time and info! And if I happen to answer any
of my own questions I will be sure to post what I learn.

Sincerely,
-brice
Massachusetts General Hospital 
Surgical Pathology, Histology

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------------------------------

Message: 4
Date: Tue, 26 Jan 2010 20:38:36 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Diff-Quik
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<abea52a61001261738q193fdfd8p44c98de3052e0b62 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I sort of apologize for this ill-natured comment, which long-term
readers of Histonet know I've made before.

I do locum tenens work, mostly in rather small pathology services -
I've worked in perhaps 60 of them in my life. Only rarely do I observe
that a histotech ever looks at a slide. I've just acquired a new
client with particularly difficult slides. The tech doesn't even have
a microscope.

The more quality assurance paperwork I have to do, the worse the slides.

The lack of feedback from pathologist to technologist is a really
widespread and serious problem. Most pathologists are completely
unwilling to take the time to do it, and the usage has never
established itself. It would be much easier if we had double headed
microscopes, which seem to be prohibited in small pathology services.

Did Edwards Deming live in vain?

Bob Richmond
Samurai Pathologist
Knoxville TN
*************************************
On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com> wrote:
> Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of  H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in your lab please. Thank you
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
> Sent: Monday, January 25, 2010 7:50 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Diff-Quik
>
> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>
> "Diff-Quik" (please note the spelling) is the trademarked name of a
> staining sequence consisting of a fixative, eosin (Diff-Quik I), and
> an azure (Diff-Quik II), done in that order in three separate
> containers.  I'm not sure who the trademark presently belongs to - it
> seems to change with the phases of the Moon.
>
> There are a number of generic equivalents, which in my personal
> experience all work as well as trademark Diff-Quik. For most ordinary
> pathology services, it isn't worthwhile to try to brew your own.
>
> I don't think I've seen bone marrow stained with such a sequence.
> Proper staining of bone marrows requires that the histotechnologist
> examine the slides under a microscope, a practice too many find
> abhorrent.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN

------------------------------

Message: 5
Date: Wed, 27 Jan 2010 12:55:11 +1100
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
Subject: RE: [Histonet] Re: Diff-Quik
To: "Robert Richmond" <rsrichmond <@t> gmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B9EAF61856077F47BF9BE2F89AFC555206853864 <@t> hedwig.nch.kids>
Content-Type: text/plain; charset="iso-8859-1"

Robert,

I agree with your comments.
There are some labs that never look at their slides (the "factories") but there are others (and this number in my experience is increasing) where the technologists often solve the problems before the slides leave the lab.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Wednesday, 27 January 2010 12:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Diff-Quik

I sort of apologize for this ill-natured comment, which long-term readers of Histonet know I've made before.

I do locum tenens work, mostly in rather small pathology services - I've worked in perhaps 60 of them in my life. Only rarely do I observe that a histotech ever looks at a slide. I've just acquired a new client with particularly difficult slides. The tech doesn't even have a microscope.

The more quality assurance paperwork I have to do, the worse the slides.

The lack of feedback from pathologist to technologist is a really widespread and serious problem. Most pathologists are completely unwilling to take the time to do it, and the usage has never established itself. It would be much easier if we had double headed microscopes, which seem to be prohibited in small pathology services.

Did Edwards Deming live in vain?

Bob Richmond
Samurai Pathologist
Knoxville TN
*************************************
On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com> wrote:
> Every slide I stain, special stains, IHC or otherwise I check under 
> the scope...I have taught all my techs to do the same, other than 
> batches of  H&E and then we check the 1st slide in each rack. I know 
> this to be a common procedure with many histology professionals. The 
> attitude can be left in your lab please. Thank you
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
> Sent: Monday, January 25, 2010 7:50 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Diff-Quik
>
> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>
> "Diff-Quik" (please note the spelling) is the trademarked name of a 
> staining sequence consisting of a fixative, eosin (Diff-Quik I), and 
> an azure (Diff-Quik II), done in that order in three separate 
> containers.  I'm not sure who the trademark presently belongs to - it 
> seems to change with the phases of the Moon.
>
> There are a number of generic equivalents, which in my personal 
> experience all work as well as trademark Diff-Quik. For most ordinary 
> pathology services, it isn't worthwhile to try to brew your own.
>
> I don't think I've seen bone marrow stained with such a sequence. 
> Proper staining of bone marrows requires that the histotechnologist 
> examine the slides under a microscope, a practice too many find 
> abhorrent.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 6
Date: Wed, 27 Jan 2010 00:22:53 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Histology Special Stains for Macrophages
To: "Willman, Sharon" <sharon.willman <@t> bms.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <fbb794cb788.4b5f875d <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII

None of the methods mentioned in the enquiry are stains for macrophages. Research workers who never took Histology 101 often stain cells of the monocyte/macrophage lineage immunohistochemically (IHC), using very expensive primary antibodies and fairly expensive kits to amplify and detect the binding sites. IHC is necessary if you must find every macrophage, including a tissue's recent monocyte immigrants that haven't yet done any work. Macrophages that have been busily eating blood are full of brown granules that don't need any staining. 

Ordinary people recognize macrophages by their appearance in sections stained with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. With the latter it's important to get the pH right - much lower for sections of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or smears (traditionally 6.8). It's all very well explained in RD Lillie & GM Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd edition, 1965, is OK too) and also in more recent textbooks including those by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course 
Yours truly.

John Kiernan
Anatomy, UWO
London, Canada
http://publish.uwo.ca/~jkiernan/bookfind.htm
= = =
----- Original Message -----
From: "Willman, Sharon" <sharon.willman <@t> bms.com>
Date: Tuesday, January 26, 2010 12:12
Subject: [Histonet] Histology Special Stains for Macrophages
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>

> Hi,
> We are needing to do a special stain for macrophages.  What 
> is the most common stain for that?  Does anyone do a Sudan 
> Black, Alcian Blue or Van Gieson for macrophages?  Any 
> information would be appreciated.
> Thanks in advance.
> Sharon
> 
> 
> ________________________________
> This message (including any attachments) may contain 
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> the individual or entity designated above. If you are not the 
> intended recipient of this message, please notify the sender 
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> disclosure, reproduction, distribution or other use of this 
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------------------------------

Message: 7
Date: Wed, 27 Jan 2010 00:56:28 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] removing trigeminal nerve and ganglion
To: Michele Wich <mwich <@t> 7thwavelabs.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <fbbba2dd6074.4b5f8f3c <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII

Why don't you say who and where you are?  

Someone must have shown you how to take out a rat's brain. This involves cutting the roots of all the cranial nerves and the pituitary stalk. You are then looking at the base of the skull, with the overlying dura mater.  The pituitary gland is in the midline, with a white centre (posterior lobe) and dark pink parts laterally (anterior lobe).  There is a broad white band on each side, extending caudally from the pituitary. This comprises the trigeminal ganglion, the extradural parts of its roots and the the nerve about to branch into its three divisions. 

With an acutely pointed scalpel, cut down on either side and below this white object lateral and posterior to the pituitary gland. Remove and fix the excavated object. This is the biggest and easiest sensory ganglion to dissect out of a rat. 

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: Michele Wich <mwich <@t> 7thwavelabs.com>
Date: Tuesday, January 26, 2010 11:15
Subject: [Histonet] removing trigeminal nerve and ganglion
To: histonet <@t> lists.utsouthwestern.edu

> Does anyone have experience/a good method for removing 
> trigeminal nerve
> and ganglion from rat intact? Any advice would be helpful.
> 

------------------------------

Message: 8
Date: Wed, 27 Jan 2010 01:06:56 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Coverslipping
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <fbb780c05f24.4b5f91b0 <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII

Is it "normal" to put the mounting medium on the slide and then apply a coverslip from above? What's wrong with putting the mounting medium on the supine coverslip and then bringing the slide down from above?  I prefer the latter method for preparations thinner than 50um.

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Date: Tuesday, January 26, 2010 14:13
Subject: [Histonet] Coverslipping
To: histonet <histonet <@t> lists.utsouthwestern.edu>

> Do any of my Esteemed Colleagues have a photo somewhere of someone
> hand-coverslipping?  I need a picture of a "normal" hand 
> coverslipping(putting coverslip ON slide instead of slide ON 
> coverslip).  Just one
> photo is all I need.  I'm trying to describe in words how 
> to do this and
> it's not easy!  Thanks in advance for your Digital Donation 
> (and I mean
> "electronic" digital, so don't give me any flak).  If you 
> can email it,
> I can pass it along.
> 
>  
> 
> Sally Breeden, HT(ASCP)
> 
> NM Dept. of Agriculture
> 
> Veterinary Diagnostic Services
> 
> PO Box 4700
> 
> Albuquerque, NM  87106
> 
> 505-841-2576
> 
>  
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Wed, 27 Jan 2010 08:15:54 -0500
From: mtitford <@t> aol.com
Subject: [Histonet] Coverslipping
To: Histonet <@t> pathology.swmed.edu
Message-ID: <8CC6D69FD7A5401-2174-11362 <@t> webmail-m099.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Before the days of automatic coverslippers, I have seen coverslipping done in large quantities from above and from below. I guess it depends what you are comfortable with. In either event, you want to get a "squeegee" effect so no air bubbles are trapped in the mountant. You need more control when coverslipping thick sections, or whole mounts. With Canada balsem, there was less problem with drying back, but that is all history now.
One technologist I saw at a London hospital would coverslip and then dip the whole slide in xylene and then wipe it off . It worked surprisingly well.

Mike Titford
USA Pathology
Mobile AL USA

------------------------------

Message: 10
Date: Wed, 27 Jan 2010 06:30:00 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Coverslipping Photo Thanks
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4D14F0FC9316DD41972D5F03C070908B02E46DDE <@t> nmdamailsvr.nmda.ad.nmsu.edu>

Content-Type: text/plain;	charset="us-ascii"

Thank you to everyone that sent pictures of hand-coverslipping!  I've
sent them on to the tech that needed some tutoring and I believe they've
been very helpful.  This Histonet thing - what a bonus!!

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

------------------------------

Message: 11
Date: Wed, 27 Jan 2010 07:06:28 -0800 (PST)
From: kristen arvidson <arvidsonkristen <@t> yahoo.com>
Subject: [Histonet] Dako vs Ventana
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <768327.23434.qm <@t> web65709.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello all,
I am trying to get a feel for some of the special stainers out there.  I came to the conclusion that it will be either Dako or Ventana.  Opinions?  Any other machines on the market that people love?  Thanks.

------------------------------

Message: 12
Date: Wed, 27 Jan 2010 10:11:48 -0500
From: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Subject: RE: [Histonet] Dako vs Ventana
To: 'kristen arvidson' <arvidsonkristen <@t> yahoo.com>, histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5A2BD13465E061429D6455C8D6B40E390E976D90C2 <@t> IBMB7Exchange.digestivespecialists.com>

Content-Type: text/plain; charset="iso-8859-1"

I love the Intellipath from BioCare. I've had mine for over a year.  It's a fully open system, it has the ability to continually add more slides to a run or start a STAT run.  You can run multiplex staining. The technical support is excellent.  It bench top size is a plus also.  

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
7415 Brandt Pike
Huber Heights, OH 45424
Phone: (937) 293-4424 ext 7118
Email: lblazek <@t> digestivespecialists.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Wednesday, January 27, 2010 10:06 AM
To: histonet
Subject: [Histonet] Dako vs Ventana

Hello all,
I am trying to get a feel for some of the special stainers out there.  I came to the conclusion that it will be either Dako or Ventana.  Opinions?  Any other machines on the market that people love?  Thanks.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 13
Date: Wed, 27 Jan 2010 07:16:06 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] Hamamatsu Nanozoomer
To: Histonet <histonet <@t> lists.utsouthwestern.edu>,
	ihcrg <@t> googlegroups.com
Message-ID: <411421.91202.qm <@t> web50304.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Everyone,

I was wondering if any of you folks had experience with the Hamamatsu Nanozoomer slide scanner.  We recently learned about it and are considering getting one instead of an Aperio slide scanner.  We plan to use Visiomorph image analysis software, so all we really need is the scanner and not all of the fancy software that these companies sell to go with their instruments.

The other labs at my company have Aperio systems and the images from the Nanozoomer would need to compatible or made to be compatabile for this to be a viable option for us (for cross-site slide conferencing purposes).  We are interested in it because this instrument can be adapted for fluorescence (with Aperio, you need to purchase 2 separate instruments; one for light microscope and one for fluorescence).

Any insights would be greatly appreciated.

Thanks,
Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

------------------------------

Message: 14
Date: Wed, 27 Jan 2010 07:18:44 -0800
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Dako vs Ventana
To: "Blazek, Linda" <lblazek <@t> digestivespecialists.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5b6eb13e1001270718k712a5987ned3c92c31c443092 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Are you really running special stains on the Intellipath?

Mark Tarango

On Wed, Jan 27, 2010 at 7:11 AM, Blazek, Linda <
lblazek <@t> digestivespecialists.com> wrote:

> I love the Intellipath from BioCare. I've had mine for over a year.  It's a
> fully open system, it has the ability to continually add more slides to a
> run or start a STAT run.  You can run multiplex staining. The technical
> support is excellent.  It bench top size is a plus also.
>
> Linda Blazek HT (ASCP)
> Manager/Supervisor
> GI Pathology of Dayton
> 7415 Brandt Pike
> Huber Heights, OH 45424
> Phone: (937) 293-4424 ext 7118
> Email: lblazek <@t> digestivespecialists.com
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
> Sent: Wednesday, January 27, 2010 10:06 AM
> To: histonet
> Subject: [Histonet] Dako vs Ventana
>
> Hello all,
> I am trying to get a feel for some of the special stainers out there.  I
> came to the conclusion that it will be either Dako or Ventana.  Opinions?
> Any other machines on the market that people love?  Thanks.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 15
Date: Wed, 27 Jan 2010 07:23:12 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] Dianova rat anti-mouse CD31
To: Histonet <histonet <@t> lists.utsouthwestern.edu>,
	ihcrg <@t> googlegroups.com
Message-ID: <990075.43781.qm <@t> web50308.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi All,

I know there was some buzz a while back about this antibody.  I have tested it on mouse FFPE xenografts, and I must say, it looks very promising.  I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF.  The NBF-fixed tumor actually looks better!

A couple of other folks that are working at the west coast sites of my company have had similar results.  We are all very encouraged.

I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 

------------------------------

Message: 16
Date: Wed, 27 Jan 2010 10:28:34 -0500
From: "Baldridge, Lee Ann" <lhadley <@t> iupui.edu>
Subject: [Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31
To: "kmerriam2003 <@t> yahoo.com" <kmerriam2003 <@t> yahoo.com>, Histonet
	<histonet <@t> lists.utsouthwestern.edu>, "ihcrg <@t> googlegroups.com"
	<ihcrg <@t> googlegroups.com>
Message-ID:
	<5E6A94F8037F4F49B738F5B6AD16952215EC2BEC2D <@t> iu-mssg-mbx09.ads.iu.edu>
Content-Type: text/plain; charset="us-ascii"

We are having good results also.
Lee Ann Baldridge
IUSM
Indpls., IN.

________________________________
From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of Kim Merriam
Sent: Wednesday, January 27, 2010 10:23 AM
To: Histonet; ihcrg <@t> googlegroups.com
Subject: [IHCRG] Dianova rat anti-mouse CD31

Hi All,

I know there was some buzz a while back about this antibody.  I have tested it on mouse FFPE xenografts, and I must say, it looks very promising.  I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF.  The NBF-fixed tumor actually looks better!

A couple of other folks that are working at the west coast sites of my company have had similar results.  We are all very encouraged.

I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone.

Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

------------------------------

Message: 17
Date: Wed, 27 Jan 2010 09:52:58 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: Re: [Histonet] Re: Diff-Quik
To: Robert Richmond <rsrichmond <@t> gmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OF7EFDD635.2FDE1EA7-ON862576B8.00568EE4-862576B8.00573F6F <@t> bhcpns.org>
Content-Type: text/plain;	charset="US-ASCII"

I'm going to have to agree with Cheryl on the comment. This may be your 
experience but I can tell you my techs always look at their stains before 
they send it on to the Pathologist. It is a requirement that they 
understand what they are looking at in order to know if it worked. Each of 
them are also trained to know all tissues microscopically and all stain 
components microscopically. That is after all the purpose of being a 
Histologist. 

I am going out on a limb here and I normally don't, but you are digging 
yourself in to a rather rude hole to insult so many professional 
Histologist. 

Just saying.............

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Robert Richmond <rsrichmond <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/26/2010 07:38 PM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Re: Diff-Quik

I sort of apologize for this ill-natured comment, which long-term
readers of Histonet know I've made before.

I do locum tenens work, mostly in rather small pathology services -
I've worked in perhaps 60 of them in my life. Only rarely do I observe
that a histotech ever looks at a slide. I've just acquired a new
client with particularly difficult slides. The tech doesn't even have
a microscope.

The more quality assurance paperwork I have to do, the worse the slides.

The lack of feedback from pathologist to technologist is a really
widespread and serious problem. Most pathologists are completely
unwilling to take the time to do it, and the usage has never
established itself. It would be much easier if we had double headed
microscopes, which seem to be prohibited in small pathology services.

Did Edwards Deming live in vain?

Bob Richmond
Samurai Pathologist
Knoxville TN
*************************************
On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com> wrote:
> Every slide I stain, special stains, IHC or otherwise I check under the 
scope...I have taught all my techs to do the same, other than batches of 
 H&E and then we check the 1st slide in each rack. I know this to be a 
common procedure with many histology professionals. The attitude can be 
left in your lab please. Thank you
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert 
Richmond
> Sent: Monday, January 25, 2010 7:50 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Diff-Quik
>
> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>
> "Diff-Quik" (please note the spelling) is the trademarked name of a
> staining sequence consisting of a fixative, eosin (Diff-Quik I), and
> an azure (Diff-Quik II), done in that order in three separate
> containers.  I'm not sure who the trademark presently belongs to - it
> seems to change with the phases of the Moon.
>
> There are a number of generic equivalents, which in my personal
> experience all work as well as trademark Diff-Quik. For most ordinary
> pathology services, it isn't worthwhile to try to brew your own.
>
> I don't think I've seen bone marrow stained with such a sequence.
> Proper staining of bone marrows requires that the histotechnologist
> examine the slides under a microscope, a practice too many find
> abhorrent.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-----------------------------------------
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This message along with any attached data, are the confidential and
proprietary communications of BHC and are intended to be received
only by the individual or individuals to whom the message has been
addressed. If the reader of this message is not the intended
recipient, please take notice that any use, copying, printing,
forwarding or distribution of this message, in any form, is
strictly prohibited and may violate State or Federal Law. If you
have received this transmission in error, please delete or destroy
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------------------------------

Message: 18
Date: Wed, 27 Jan 2010 10:09:20 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Re: Diff-Quik
To: <Kim.Donadio <@t> bhcpns.org>,	"Robert Richmond" <rsrichmond <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A3D20 <@t> is-e2k3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

Give it a rest!  Dr. Richmond 'sort of apologized' for his comments. He
does explain his views and why he stated them. That should be the end of
it!

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kim.Donadio <@t> bhcpns.org
Sent: Wednesday, January 27, 2010 9:53 AM
To: Robert Richmond
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Diff-Quik

I'm going to have to agree with Cheryl on the comment. This may be your 
experience but I can tell you my techs always look at their stains
before 
they send it on to the Pathologist. It is a requirement that they 
understand what they are looking at in order to know if it worked. Each
of 
them are also trained to know all tissues microscopically and all stain 
components microscopically. That is after all the purpose of being a 
Histologist. 

I am going out on a limb here and I normally don't, but you are digging 
yourself in to a rather rude hole to insult so many professional 
Histologist. 

Just saying.............

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Robert Richmond <rsrichmond <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/26/2010 07:38 PM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Re: Diff-Quik

I sort of apologize for this ill-natured comment, which long-term
readers of Histonet know I've made before.

I do locum tenens work, mostly in rather small pathology services - I've
worked in perhaps 60 of them in my life. Only rarely do I observe that a
histotech ever looks at a slide. I've just acquired a new client with
particularly difficult slides. The tech doesn't even have a microscope.

The more quality assurance paperwork I have to do, the worse the slides.

The lack of feedback from pathologist to technologist is a really
widespread and serious problem. Most pathologists are completely
unwilling to take the time to do it, and the usage has never established
itself. It would be much easier if we had double headed microscopes,
which seem to be prohibited in small pathology services.

Did Edwards Deming live in vain?

Bob Richmond
Samurai Pathologist
Knoxville TN
*************************************
On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com>
wrote:
> Every slide I stain, special stains, IHC or otherwise I check under 
> the
scope...I have taught all my techs to do the same, other than batches of

 H&E and then we check the 1st slide in each rack. I know this to be a 
common procedure with many histology professionals. The attitude can be 
left in your lab please. Thank you
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert 
Richmond
> Sent: Monday, January 25, 2010 7:50 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Diff-Quik
>
> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>
> "Diff-Quik" (please note the spelling) is the trademarked name of a 
> staining sequence consisting of a fixative, eosin (Diff-Quik I), and 
> an azure (Diff-Quik II), done in that order in three separate 
> containers.  I'm not sure who the trademark presently belongs to - it 
> seems to change with the phases of the Moon.
>
> There are a number of generic equivalents, which in my personal 
> experience all work as well as trademark Diff-Quik. For most ordinary 
> pathology services, it isn't worthwhile to try to brew your own.
>
> I don't think I've seen bone marrow stained with such a sequence. 
> Proper staining of bone marrows requires that the histotechnologist 
> examine the slides under a microscope, a practice too many find 
> abhorrent.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-----------------------------------------
All electronic data transmissions originating from or sent to Baptist
Health Care Corporation (BHC) are subject to monitoring. This message
along with any attached data, are the confidential and proprietary
communications of BHC and are intended to be received only by the
individual or individuals to whom the message has been addressed. If the
reader of this message is not the intended recipient, please take notice
that any use, copying, printing, forwarding or distribution of this
message, in any form, is strictly prohibited and may violate State or
Federal Law. If you have received this transmission in error, please
delete or destroy all copies of this message.  For questions, contact
the BHC Privacy Officer at (850) 434-4472.  Rev.10/07.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 19
Date: Wed, 27 Jan 2010 10:11:00 -0600
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: RE: [Histonet] Dako vs Ventana
To: 'kristen arvidson' <arvidsonkristen <@t> yahoo.com>, histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<8F0EE4144E8E2F4CA1F6B051A2E5BFEE328A5621 <@t> EXCHMBC2.ad.ah.local>
Content-Type: text/plain; charset="iso-8859-1"

We find the Ventana very easy and it takes a very short time to load/set up.  We did demo the DAKO and my techs felt it was too easy to make errors and took too long to get a run going.
Jan Mahoney
Omaha, NE

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of kristen arvidson
Sent: Wednesday, January 27, 2010 9:06 AM
To: histonet
Subject: [Histonet] Dako vs Ventana

Hello all,
I am trying to get a feel for some of the special stainers out there.  I came to the conclusion that it will be either Dako or Ventana.  Opinions?  Any other machines on the market that people love?  Thanks.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 20
Date: Wed, 27 Jan 2010 10:17:18 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: RE: [Histonet] Re: Diff-Quik
To: "Mike Pence" <mpence <@t> grhs.net>
Cc: histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu,	Robert Richmond
	<rsrichmond <@t> gmail.com>
Message-ID:
	<OF9FDF436A.4DB86F5F-ON862576B8.00592E4D-862576B8.00597974 <@t> bhcpns.org>
Content-Type: text/plain; charset="US-ASCII"

Thanks for your profound attitude. A "sort of apology" is like a boat that 
"sort of floats" . I am surprised at the unprofessional comments that get 
sent to my desk from here every day. Not sure if it is really worth it. 

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

"Mike Pence" <mpence <@t> grhs.net> 
01/27/2010 10:09 AM

To
<Kim.Donadio <@t> bhcpns.org>, "Robert Richmond" <rsrichmond <@t> gmail.com>
cc
<histonet <@t> lists.utsouthwestern.edu>, 
<histonet-bounces <@t> lists.utsouthwestern.edu>
Subject
RE: [Histonet] Re: Diff-Quik

Give it a rest!  Dr. Richmond 'sort of apologized' for his comments. He
does explain his views and why he stated them. That should be the end of
it!

Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Kim.Donadio <@t> bhcpns.org
Sent: Wednesday, January 27, 2010 9:53 AM
To: Robert Richmond
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Diff-Quik

I'm going to have to agree with Cheryl on the comment. This may be your 
experience but I can tell you my techs always look at their stains
before 
they send it on to the Pathologist. It is a requirement that they 
understand what they are looking at in order to know if it worked. Each
of 
them are also trained to know all tissues microscopically and all stain 
components microscopically. That is after all the purpose of being a 
Histologist. 

I am going out on a limb here and I normally don't, but you are digging 
yourself in to a rather rude hole to insult so many professional 
Histologist. 

Just saying.............

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Robert Richmond <rsrichmond <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/26/2010 07:38 PM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Re: Diff-Quik

I sort of apologize for this ill-natured comment, which long-term
readers of Histonet know I've made before.

I do locum tenens work, mostly in rather small pathology services - I've
worked in perhaps 60 of them in my life. Only rarely do I observe that a
histotech ever looks at a slide. I've just acquired a new client with
particularly difficult slides. The tech doesn't even have a microscope.

The more quality assurance paperwork I have to do, the worse the slides.

The lack of feedback from pathologist to technologist is a really
widespread and serious problem. Most pathologists are completely
unwilling to take the time to do it, and the usage has never established
itself. It would be much easier if we had double headed microscopes,
which seem to be prohibited in small pathology services.

Did Edwards Deming live in vain?

Bob Richmond
Samurai Pathologist
Knoxville TN
*************************************
On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com>
wrote:
> Every slide I stain, special stains, IHC or otherwise I check under 
> the
scope...I have taught all my techs to do the same, other than batches of

 H&E and then we check the 1st slide in each rack. I know this to be a 
common procedure with many histology professionals. The attitude can be 
left in your lab please. Thank you
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert 
Richmond
> Sent: Monday, January 25, 2010 7:50 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Diff-Quik
>
> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>
> "Diff-Quik" (please note the spelling) is the trademarked name of a 
> staining sequence consisting of a fixative, eosin (Diff-Quik I), and 
> an azure (Diff-Quik II), done in that order in three separate 
> containers.  I'm not sure who the trademark presently belongs to - it 
> seems to change with the phases of the Moon.
>
> There are a number of generic equivalents, which in my personal 
> experience all work as well as trademark Diff-Quik. For most ordinary 
> pathology services, it isn't worthwhile to try to brew your own.
>
> I don't think I've seen bone marrow stained with such a sequence. 
> Proper staining of bone marrows requires that the histotechnologist 
> examine the slides under a microscope, a practice too many find 
> abhorrent.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

-----------------------------------------
All electronic data transmissions originating from or sent to Baptist
Health Care Corporation (BHC) are subject to monitoring. This message
along with any attached data, are the confidential and proprietary
communications of BHC and are intended to be received only by the
individual or individuals to whom the message has been addressed. If the
reader of this message is not the intended recipient, please take notice
that any use, copying, printing, forwarding or distribution of this
message, in any form, is strictly prohibited and may violate State or
Federal Law. If you have received this transmission in error, please
delete or destroy all copies of this message.  For questions, contact
the BHC Privacy Officer at (850) 434-4472.  Rev.10/07.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 21
Date: Wed, 27 Jan 2010 10:25:16 -0600
From: "Cory Collins" <cory.collins <@t> DHAT.com>
Subject: [Histonet] Pathology Laboratory Manager Opening in Houston,
	Texas
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<A7E71FD882433346B99787DEA2DEEA520480A3B7 <@t> exchange.DHAT.COM>
Content-Type: text/plain;	charset="us-ascii"

SightLine is expanding their service lines and is seeking a pathology
laboratory manager who will be responsible for the pathology laboratory
services from the design and construction of the new facility, equipment
purchasing, hiring staff, policies and procedures, certification and
licensure.  The ideal candidate must have managed laboratory staff and
have been through CLIA certification.  They must also have the ability
to step-in as a laboratory technician should the situation demanded it.

The new facility will be located on South Main near the Reliant Stadium
in Houston, Texas 

Reports to Vice President of Operations.

Education: Bachelor Degree in Science with appropriate on the job
training. 

Please indicate your interest by sending emails with resumes attached to
hr <@t> sightlinehealth.com

------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 74, Issue 32
****************************************