[Histonet] Re: Diff-Quik

Wed Jan 27 09:52:58 CST 2010

I'm going to have to agree with Cheryl on the comment. This may be your 
experience but I can tell you my techs always look at their stains before 
they send it on to the Pathologist. It is a requirement that they 
understand what they are looking at in order to know if it worked. Each of 
them are also trained to know all tissues microscopically and all stain 
components microscopically. That is after all the purpose of being a 
Histologist. 

I am going out on a limb here and I normally don't, but you are digging 
yourself in to a rather rude hole to insult so many professional 
Histologist. 

Just saying.............

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Robert Richmond <rsrichmond <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/26/2010 07:38 PM

To
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] Re: Diff-Quik

I sort of apologize for this ill-natured comment, which long-term
readers of Histonet know I've made before.

I do locum tenens work, mostly in rather small pathology services -
I've worked in perhaps 60 of them in my life. Only rarely do I observe
that a histotech ever looks at a slide. I've just acquired a new
client with particularly difficult slides. The tech doesn't even have
a microscope.

The more quality assurance paperwork I have to do, the worse the slides.

The lack of feedback from pathologist to technologist is a really
widespread and serious problem. Most pathologists are completely
unwilling to take the time to do it, and the usage has never
established itself. It would be much easier if we had double headed
microscopes, which seem to be prohibited in small pathology services.

Did Edwards Deming live in vain?

Bob Richmond
Samurai Pathologist
Knoxville TN
*************************************
On Tue, Jan 26, 2010 at 9:03 AM, Cheri Miller <cmiller <@t> physlab.com> wrote:
> Every slide I stain, special stains, IHC or otherwise I check under the 
scope...I have taught all my techs to do the same, other than batches of 
 H&E and then we check the 1st slide in each rack. I know this to be a 
common procedure with many histology professionals. The attitude can be 
left in your lab please. Thank you
>
> Cheryl Miller HT ASCP CM
> Histology Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4148
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert 
Richmond
> Sent: Monday, January 25, 2010 7:50 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Diff-Quik
>
> Thanks, John Kiernan, for your explanation of Romanovsky stains.
>
> "Diff-Quik" (please note the spelling) is the trademarked name of a
> staining sequence consisting of a fixative, eosin (Diff-Quik I), and
> an azure (Diff-Quik II), done in that order in three separate
> containers.  I'm not sure who the trademark presently belongs to - it
> seems to change with the phases of the Moon.
>
> There are a number of generic equivalents, which in my personal
> experience all work as well as trademark Diff-Quik. For most ordinary
> pathology services, it isn't worthwhile to try to brew your own.
>
> I don't think I've seen bone marrow stained with such a sequence.
> Proper staining of bone marrows requires that the histotechnologist
> examine the slides under a microscope, a practice too many find
> abhorrent.
>
> Bob Richmond
> Samurai Pathologist
> Knoxville TN

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