[Histonet] RE: [IHCRG] Dianova rat anti-mouse CD31

[Baldridge, Lee Ann]

We are having good results also.
Lee Ann Baldridge
IUSM
Indpls., IN.

________________________________
From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of Kim Merriam
Sent: Wednesday, January 27, 2010 10:23 AM
To: Histonet; ihcrg <@t> googlegroups.com
Subject: [IHCRG] Dianova rat anti-mouse CD31

Hi All,

I know there was some buzz a while back about this antibody.  I have tested it on mouse FFPE xenografts, and I must say, it looks very promising.  I compared 2 pieces of the same mouse xenograft; one half of the tumor was fixed in IHC-Zinc (ZInc-Tris) and stained with the BD rat anti-mouse CD31 and the other half of the tumor was fixed in NBF.  The NBF-fixed tumor actually looks better!

A couple of other folks that are working at the west coast sites of my company have had similar results.  We are all very encouraged.

I still need to do a bit more testing with some other tissues (and perhaps some additional titrations), but I thought I would pass this along to everyone.

Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA