[Histonet] Simple dye for cornea and sclera.

[Reno Genest]

Hello everyone,

I am trying to reconstruct the 3D geometry of the cornea and sclera of  
a chicken eye. I embedded and froze an eye using OCT and CO2 liquid  
withdrawal and sliced it using a microtome. I took pictures from the  
top of the eye (not the sections but what is left after slicing) using  
a digital camera. The problem is that the sclera and cornea are  
difficult to see.

Is there a dye I could use before taking a picture that can be applied  
in one step and do not require a lengthy protocol? I cannot afford to  
wash the eye with tap water because it is still frozen on the  
microtome. Also, is anyone aware of a dye that would permeate through  
the sclera and cornea so that I could just dip the eye in before  
slicing it?

Thank you.


Reno Genest
MSc. candidate
Mechanical engineering
University of Waterloo