[Histonet] Wright-Giemsa stain

[DKBoyd <@t> chs.net]

We also do the bone marrow assisting/staining etc.  We stain our smears on 
a Wescor AeroSpray  slide stainer.  We use the Rapid program which is a 5 
min program.  But, we have to stain them twice to get the correct 
intensity that our pathologist prefer.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







"Marshall, Kimberly" <Kimberly.Marshall <@t> ahss.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/22/2010 04:27 PM

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histonet <@t> lists.utsouthwestern.edu
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Subject
[Histonet] Wright-Giemsa stain






I am from a small Hospital where due to the size, Histology does the
Bone Marrow's.  We go to the procedure, make the smears, as well as do
the Wright-Giemsa stain.   I am having a hard time getting the Wright's
stain dark enough for my Pathologist.   I have added stain time, checked
the pH of my stain, tried different rinse's and buffers, but still not
getting it dark enough.  I do go back to the stain for 45 mins or more
and that will get it at least close to what they want.  Is there anyone
out there that can give me some advise on this stain?   Thanks in
advance for your time

 

Kimberly


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