[Histonet] Re: Wright-Giemsa stain

[Liz Chlipala]

One other thing to be aware of is any formaldehyde fumes, you need to
keep the smear samples away from formalin as much as possible.  If the
bone marrow smears come in contact with formalin fumes they will not
stain properly.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Saturday, January 23, 2010 1:27 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Wright-Giemsa stain

Kimberly Marshall asks: >>I am from a small hospital where due to the
size, Histology does the
Bone Marrows. We go to the procedure, make the smears, as well as do
the Wright-Giemsa stain.  I am having a hard time getting the Wright's
stain dark enough for my pathologist. I have added stain time, checked
the pH of my stain, tried different rinses and buffers, but still not
getting it dark enough. I do go back to the stain for 45 minutes or
more and that will get it at least close to what they want. Is there
anyone out there that can give me some advise on this stain?<<

There are many possibilities. First thing I'd do is get a new bottle
of stain, preferably from a different source. Very small amounts of
water contaminating the stock bottle are ruinous.

Bob Richmond
Samurai Pathologist
Knoxville TN

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