[Histonet] Joel - re: tendons

[Liz Chlipala]

Vicki is correct for larger samples I process manually with graded
alcohols - overnight for each step till I get to the last 100% alcohol,
I do 3 100% for larger samples (we process large 2 x 3 tissue both soft
tissue and bone routinely).  You might think that these tissues will be
over processed but we have never really experienced that.  What you
don't want to have is an under processed sample.  I have a processing
cycle on the processor that's 6 hours per station, for the last alcohol,
xylenes and paraffins (I also use 3 xylenes and 4 paraffin stations).
Vicki is correct - tendon can be challenging.  The reason we manually
process is that we can't tie up our tissue processor that much for these
samples.  We only have one.  But we have lots of different processing
cycles for all types of tissue.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vicki
Kalscheur
Sent: Friday, January 22, 2010 11:36 AM
To: Histonet Discussion
Subject: [Histonet] Joel - re: tendons

Joel:  Increase all stations of processing time, as Liz suggested. For
large sections of tendon, or when looking at length with both tendon
attachments, I process by hand and soak in paraffin changes over-night
(oven). Unless you have a bone attachment, decalcification should be
unnecessary.  Tendons are challenging. The smaller the tissue size, the
easier to work with and to get thinner, better quality sections. Vicki
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