[Histonet] Tendon processing question

[Sherwood, Margaret]

Liz,

In that regard, we just received mice tibia in formalin for IHC.  We need to do
decalcification.   Do you recommend formic acid (could you give us the
procedure)and how long in the formic acid?  We do very little decal and have had
problems in the past with it being incomplete.

Any help would be greatly appreciated!

Thanks!
Peggy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Thursday, January 21, 2010 2:45 PM
To: Joel Reichensperger; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Tendon processing question

I would use formic acid as a decalcification agent, it works well with
IHC.  We have processed quite a few tendons and we find that you also
need a longer processing cycle, 1 - 1.5 hours per station.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joel
Reichensperger
Sent: Thursday, January 21, 2010 12:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Tendon processing question

We are looking for the best way to process tendon (specifically rabbit) 
that has been severed and then repaired. Currently they are removing the

tendon, rinsing it in PBS and then putting it in a cassette in formalin.

The problem I am having is that there appears to be some calcification 
or some other hardening at the area of the repair which is causing the 
sections to shred when they are cut. We are thinking about using a 
decalcification agent, but not sure if one is better than another for 
this type of tissue. We intend to due IHC on the slides once they are 
cut. Does anyone have any experience with this problem and would be 
willing to help me out?

Thanks,

Joel Reichensperger

-- 
Joel Reichensperger
Researcher II
Southern Illinois University 
Plastic Surgery Institute
jreichensperger <@t> siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


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