[Histonet] Tendon processing question

[Joel Reichensperger]

We are looking for the best way to process tendon (specifically rabbit) 
that has been severed and then repaired. Currently they are removing the 
tendon, rinsing it in PBS and then putting it in a cassette in formalin. 
The problem I am having is that there appears to be some calcification 
or some other hardening at the area of the repair which is causing the 
sections to shred when they are cut. We are thinking about using a 
decalcification agent, but not sure if one is better than another for 
this type of tissue. We intend to due IHC on the slides once they are 
cut. Does anyone have any experience with this problem and would be 
willing to help me out?

Thanks,

Joel Reichensperger

-- 
Joel Reichensperger
Researcher II
Southern Illinois University 
Plastic Surgery Institute
jreichensperger <@t> siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)