[Histonet] EBER ISH problems

[Josie Britton]

Try switching your DAB kit to Refined Red, this helped us.  We also run
a negative RNA and positive RNA on the patient also.

 

Josie Britton HT

Cheshire Medical Center

Keene, NH

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Goodacre, Suzanne
Sent: Wednesday, January 20, 2010 11:20 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] EBER ISH problems

 

Hello,

        Our Histo lab currently uses the Leica Microsystems Bond
instrument for EBER ISH.  We noticed that eosinophils readily pick up
the DAB detection reagents that we use.  I've attempted to alter the
EBER ISH protocol on the BOND so that the eosinophils don't take on the
detection reagents but I'm not having much luck.  Does anyone have any
tips on how to prevent or avoid the problem?

 

Suzanne Goodacre

Research Technologist

Micro Molecular Lab

Children's Hospital of Wisconsin

414-266-2709

 

 

_______________________________________________

Histonet mailing list

Histonet <@t> lists.utsouthwestern.edu

http://lists.utsouthwestern.edu/mailman/listinfo/histonet


CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message.