[Histonet] EBER ISH problems

[Goodacre, Suzanne]

Hello,
        Our Histo lab currently uses the Leica Microsystems Bond instrument for EBER ISH.  We noticed that eosinophils readily pick up the DAB detection reagents that we use.  I've attempted to alter the EBER ISH protocol on the BOND so that the eosinophils don't take on the detection reagents but I'm not having much luck.  Does anyone have any tips on how to prevent or avoid the problem?

Suzanne Goodacre
Research Technologist
Micro Molecular Lab
Children's Hospital of Wisconsin
414-266-2709