[Histonet] Nuclei staining

[John Kiernan]

With the methods you list, you should easily be able to get selective nuclear staining with haemalum ("haematoxylin") or with Weigert's haematoxylin. The other traditional method, which is specific for DNA, is the Feulgen reaction. All these methods are in every book of staining techniques
 
John Kiernan.
Anatomy, UWO
London, Canada
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----- Original Message -----
From: "Tilston, Valerie" <V.Tilston <@t> liverpool.ac.uk>
Date: Friday, January 15, 2010 6:08
Subject: [Histonet] Nuclei staining
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>

> Hi,
> 
> I'm trying to find a  stain for nuclei only (without a high 
> background stain) so that I am able to count the number of 
> nuclei present in paraffin fixed livers.  I appreciate that 
> I could do DAPI immunofluorescence or IHC etc but does anyone 
> know of any basic histology stains/protocols that give good 
> results? I've already tried haematoxyin, nuclear fast red, 
> carbol fuschin &  Weigerts A+B.....
> 
> Many thanks,
> 
> Val
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