[Histonet] Nuclei staining

[Tilston, Valerie]

Hi,

I'm trying to find a  stain for nuclei only (without a high background stain) so that I am able to count the number of nuclei present in paraffin fixed livers.  I appreciate that I could do DAPI immunofluorescence or IHC etc but does anyone know of any basic histology stains/protocols that give good results? I've already tried haematoxyin, nuclear fast red, carbol fuschin &  Weigerts A+B.....

Many thanks,

Val