[Histonet] Eliminating the edge effect in IHC/IF

[Karen Cai]

Hi,=20
Thank you very much for your input. Could you describe more with the
floating section method?  I never did it before.
=20
Best,
Karen
=20
-----Original Message-----
From: TF [mailto:tifei <@t> foxmail.com]=20
Sent: Wednesday, January 13, 2010 6:11 PM
To: Rene J Buesa; histonet; Karen Cai
Subject: Re: Re: [Histonet] Eliminating the edge effect in IHC/IF
=20
Hi all
i believe it is due to the wash + antibody accumulation problem happened
between the edge of section and the adhesive slide.
=20
Even you have circled the section with IHC pen(DAKO), and flattened all
soultions evenly, it improved but did not disappear
=20
if you stain the section using "floating section" method, no edge effect
now.
=20
=20
2010-01-14=20
  _____ =20

TF=20
  _____ =20

=B7=A2=BC=FE=C8=CB=A3=BA Rene J Buesa=20
=B7=A2=CB=CD=CA=B1=BC=E4=A3=BA 2010-01-12  13:30:49=20
=CA=D5=BC=FE=C8=CB=A3=BA histonet; Karen Cai=20
=B3=AD=CB=CD=A3=BA=20
=D6=F7=CC=E2=A3=BA Re: [Histonet] Eliminating the edge effect in IHC/IF=20
Usually that is the result of incomplete fixation. Check your fixation
protocol.
Ren=A8=A6 J.
--- On Mon, 1/11/10, Karen Cai <kcai <@t> prosci-inc.com> wrote:
From: Karen Cai <kcai <@t> prosci-inc.com>
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM
Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
Your kind help is greatly appreciated,
Thanks in advance,
Best,
Karen
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
<http://www.prosci-inc.com> www.prosci-inc.com
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     =20
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