Re: Re: [Histonet] Eliminating the edge effect in IHC/IF

[TF]

Hi all
i believe it is due to the wash + antibody accumulation problem happened between the edge of section and the adhesive slide.

Even you have circled the section with IHC pen(DAKO), and flattened all soultions evenly, it improved but did not disappear

if you stain the section using "floating section" method, no edge effect now.


2010-01-14 



TF 



发件人： Rene J Buesa 
发送时间： 2010-01-12  13:30:49 
收件人： histonet; Karen Cai 
抄送： 
主题： Re: [Histonet] Eliminating the edge effect in IHC/IF 
 
Usually that is the result of incomplete fixation. Check your fixation protocol.
René J.
--- On Mon, 1/11/10, Karen Cai <kcai <@t> prosci-inc.com> wrote:
From: Karen Cai <kcai <@t> prosci-inc.com>
Subject: [Histonet] Eliminating the edge effect in IHC/IF
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, January 11, 2010, 2:00 PM
Hi,
I have a question for the generous input. When I do the IHC or IF, it
seems very common that the intensity of the edge area of the tissue is
always stronger than the central tissue part. Is it possible to
eliminate this and make the staining evenly distributed around the whole
tissue section?
Your kind help is greatly appreciated,
Thanks in advance,
Best,
Karen
Karen Cai
Research Scientist
Prosci Incorporated
(858) 513-2638 x 204
(858) 513-2692 Fax
<http://www.prosci-inc.com> www.prosci-inc.com
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