[Histonet] Eliminating the edge effect in IHC/IF
Colleen Forster
cforster <@t> umn.edu
Mon Jan 11 14:00:23 CST 2010
This can also be a fixation issue. The edge fixes faster than the center....
Colleen Forster
V. Neubert wrote:
> Is it just unevenly stained? Or are your sections unevenly thick!?
>
>
>
>> Hi,
>> I have a question for the generous input. When I do the IHC or IF, it
>> seems very common that the intensity of the edge area of the tissue is
>> always stronger than the central tissue part. Is it possible to
>> eliminate this and make the staining evenly distributed around the whole
>> tissue section?
>>
>> Your kind help is greatly appreciated,
>>
>>
>> Thanks in advance,
>>
>> Best,
>> Karen
>>
>> Karen Cai
>> Research Scientist
>> Prosci Incorporated
>> (858) 513-2638 x 204
>> (858) 513-2692 Fax
>> <http://www.prosci-inc.com> www.prosci-inc.com
>>
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>
>
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