[Histonet] Eliminating the edge effect in IHC/IF

[Colleen Forster]

This can also be a fixation issue. The edge fixes faster than the center....

Colleen Forster


V. Neubert wrote:
> Is it just unevenly stained? Or are your sections unevenly thick!?
>
>
>   
>> Hi,
>> I have a question for the generous input. When I do the IHC or IF, it
>> seems very common that the intensity of the edge area of the tissue is
>> always stronger than the central tissue part. Is it possible to
>> eliminate this and make the staining evenly distributed around the whole
>> tissue section?
>>  
>> Your kind help is greatly appreciated,
>>  
>>  
>> Thanks in advance,
>>  
>> Best,
>> Karen
>>  
>> Karen Cai
>> Research Scientist
>> Prosci Incorporated
>> (858) 513-2638 x 204
>> (858) 513-2692 Fax
>>  <http://www.prosci-inc.com> www.prosci-inc.com
>>  
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