[Histonet] Eliminating the edge effect in IHC/IF

[V. Neubert]

Is it just unevenly stained? Or are your sections unevenly thick!?


> Hi,
> I have a question for the generous input. When I do the IHC or IF, it
> seems very common that the intensity of the edge area of the tissue is
> always stronger than the central tissue part. Is it possible to
> eliminate this and make the staining evenly distributed around the whole
> tissue section?
>  
> Your kind help is greatly appreciated,
>  
>  
> Thanks in advance,
>  
> Best,
> Karen
>  
> Karen Cai
> Research Scientist
> Prosci Incorporated
> (858) 513-2638 x 204
> (858) 513-2692 Fax
>  <http://www.prosci-inc.com> www.prosci-inc.com
>  
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