[Histonet] Podoplanin D2-40

[Carol Bryant]

I am using Podoplanin, D2-40 on cytology cases.  We use a mesothelioma tissue for the control and the control performs appropriately, but our cell blocks are staining very faintly.  We stain on the Ventana Benchmark using the following protocol:  Depar, standard CC1, antibody for 32 min, amplify selected, counterstain, and post counterstain.   I am thinking the standard CC1 may be too harsh for the cell blocks and maybe cutting it back to mild treatment may help. Any other suggestions to boost the staining intensity of the podoplanin on the cell blocks?
Thank you in advance for your thoughts and input.

Carol

Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com



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