[Histonet] Special stains

[Kim.Donadio <@t> bhcpns.org]

In the end it is still a new instrument. It should be validated for all 
the stains you do on it. This would seem like best practice to me. 



Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996



Rene J Buesa <rjbuesa <@t> yahoo.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/05/2010 11:38 AM

To
"'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>, 
Dorothy LWebb <Dorothy.L.Webb <@t> HealthPartners.Com>
cc

Subject
Re: [Histonet] Special stains






Dorothy:
I would NOT do it.
If you do not change the procedure it does not really matter which auto 
stainer you use. Your "validation" is the reaction of your control that 
should react as before.
As a matter of fact, I would be more inclined to made a validation if you 
change your control rather than if you change the auto stainer.
If, for example, you are doing IHC and your dilution has been "fine tuned" 
for a given tissue control and you change the tissue, you will need to 
"fine tune" the dilution to have the same reaction strength of the epitope 
in the new control, but you will not have to change your procedure. That 
is why I always used IHC internal controls, and by using "normal strength 
epitopes" I don't have to keep validating.
That is also why I always shied away from using pathological cases to use 
as epitope controls.
So, I would not advise you to go through validation because of changing 
the auto stainer, as long as the procedure is the same.
René J.

--- On Tue, 1/5/10, Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com> 
wrote:


From: Webb, Dorothy L <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Special stains
To: "'histonet <@t> lists.utsouthwestern.edu'" 
<histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, January 5, 2010, 12:23 PM


We are switching automated special stain equipment to a different vendor 
and am wondering if we have to validate all of the special stains we do.  
I am from the "old school" and the validation process, other than IHC, is 
not my forte!!  Any advice would be appreciated, so, thanks ahead of 
time!!

Dorothy Webb, Ht (ASCP)
Regions Histology Technical Supervisor
651-254-2962



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