[Histonet] mouse Cytokine ICC/IHC protocol help.

[Adam .]

I've managed to get one chemokine (SDF-1) to work on FFPE sections. The key
was using the tyramide amplification system from Perkin Elmer. It's a dirty
staining because it's secreted so you see staining bound up randomly in
noncellular extracellular matrix but you do see it concentrated in some
cells. Since it's not easy, our lab tries to avoid doing this type of
delicate work and instead do ELISAs and intracellular flow cytometry.

Adam

On Mon, Jan 4, 2010 at 12:41 PM, Jamie E Erickson <jamie.erickson <@t> abbott.com
> wrote:

> Hi All,  Happy New Year...
>
> As the new year is upon us I and my fellow histologist feel compelled to
> attempt IHC on mouse tissue again for the detection of various cytokines.
> As I have not had much success in this area in the past I thought I would
> ask if there is anyone out there that you know that may help us on our
> Journey..
>
> We want to try to detect cytokine (TNF,IL-1,IL-13) along with our soluble
> targets in mouse and rat tissues.
> Ideally we want to use paraffin sections so we will explore
> fixations/processing schedules..and any other voodoo solutions that  might
> work.
>
> If you or someone you know has a good source of papers/ protocol we might
> download we would love to have them..
>
> As we have the ability to use mice and rats we will be
> simulating/overexpression of cytokines in-vivo and taking tissues at
> necropsies in various fixations.
>
> If you can help please let me know.
>
> Thanks and Happy new Year.
>
> Jamie Erickson
> Abbott Labs.
> Scientist II, M.S. HTL (ASCP)
> Discovery Safety, Metabolism & Pharmacokinetics
> Phone 508-688-3134
> FAX 508-793-4895
> jamie.erickson <@t> abbott.com
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