[Histonet] mouse Cytokine ICC/IHC protocol help.

[Jamie E Erickson]

Hi All,  Happy New Year...

As the new year is upon us I and my fellow histologist feel compelled to 
attempt IHC on mouse tissue again for the detection of various cytokines. 
As I have not had much success in this area in the past I thought I would 
ask if there is anyone out there that you know that may help us on our 
Journey..

We want to try to detect cytokine (TNF,IL-1,IL-13) along with our soluble 
targets in mouse and rat tissues. 
Ideally we want to use paraffin sections so we will explore 
fixations/processing schedules..and any other voodoo solutions that  might 
work. 

If you or someone you know has a good source of papers/ protocol we might 
download we would love to have them..

As we have the ability to use mice and rats we will be 
simulating/overexpression of cytokines in-vivo and taking tissues at 
necropsies in various fixations.

If you can help please let me know.

Thanks and Happy new Year.

Jamie Erickson
Abbott Labs.
Scientist II, M.S. HTL (ASCP)
Discovery Safety, Metabolism & Pharmacokinetics
Phone 508-688-3134
FAX 508-793-4895
jamie.erickson <@t> abbott.com