[Histonet] Cutting Frozen Lung

[Watson, Linda]

I always perfuse/inflate my lung samples with dilute OCT before freezing. It does make the cutting much easier and the morphology is pretty good considering that it is a frozen section.

Good luck,
Linda

>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-
>bounces <@t> lists.utsouthwestern.edu] On Behalf Of Greg Dobbin
>Sent: Monday, January 04, 2010 10:44 AM
>To: histonet <@t> lists.utsouthwestern.edu; meghan.tucker <@t> yahoo.com
>Subject: Re: [Histonet] Cutting Frozen Lung
>
>Hi Meghan,
>Healthy lung is terrible to cut frozen. All those air spaces collapse
>on sectioning. Unhealthy lung on the other hand is a dream to cut! I
>have heard it suggested to perfuse the lungs with dilute frozen cutting
>compound (eg OCT or similar) prior to selecting the piece(s) for frozen
>sectioning. In theory it should work well but I have not tried it
>myself. Otherwise, a quick swipe of the cutting surface with your thumb
>just before sectioning might help a little (maximizing the amount of
>viewable tissue in the section) but your sections (of healthy lung)will
>never be "beautiful" in my experience.
>good luck.
>Greg
>
>Greg Dobbin, R.T.
>Chief Technologist, Anatomic Pathology
>Dept. of Laboratory Medicine,
>Queen Elizabeth Hospital,
>P.O. Box 6600
>Charlottetown, PE    C1A 8T5
>Phone: (902) 894-2337
>Fax: (902) 894-2385
>
>"I find that the harder I work, the
>more luck I seem to have."
>- Thomas Jefferson
>
>
>>>> Meghan Tucker <meghan.tucker <@t> yahoo.com> 1/4/2010 11:23:01 AM >>>
>Hello!
>
>I am looking for some helpful hints for cutting frozen lung tissue. I
>have tried just about everything I can think of!
>
>Thanks!
>
>
>
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